不同前处理方式对电感耦合等离子体发射光谱法测定饲料中4种微量元素的影响

Effects of different pretreatment methods on the determination of 4 kinds of trace elements in feeds by inductively coupled plasma atomic emission spectrometry

中文关键词:  微波消解  湿法消解  饲料  微量元素  电感耦合等离子体发射光谱法

英文关键词:microwave digestion  wet digestion  feed  trace elements  inductively coupled plasma atomic emission spectrometry

中文摘要:

      目的 比较分析微波消解法和湿法消解法对电感耦合等离子体发射光谱法测定饲料中铜、铁、锰、锌的影响。方法 以微波消解法处理测定的结果为对照, 通过不同消解方式处理饲料和标准物质, 测定其中铜、铁、锰、锌的含量, 比较分析湿法消解对饲料中铜、铁、锰、锌含量测定的影响。结果 仪器调制最优条件, 在0.02~20.0 mg/L(铜、铁、锰、锌)的浓度范围内, 4种微量元素测量线性良好, 相关系数均大于0.9999, 检出限为0.025~0.160 mg/kg, 定量限为0.083~0.533 mg/kg。微波消解法和湿法消解法消化饲料样品均能获得令人满意的回收率和重复性, 回收率为95.7%~104.9%, 相对标准偏差均小于3%。在实际样品测量中, 对比分析发现微波法与湿法对4种元素的测量结果无显著差异。结论 湿法消解在日常实验中可用于替代微波法进行样品前处理。

英文摘要:

      Objective To compare and analyze the influence of microwave digestion and wet digestion on the determination results of copper, iron, manganese and zinc in feeds by inductively coupled plasma atomic emission spectrometry. Methods The content of copper, iron, manganese and zinc in feeds and reference substances was determined by different digestion methods using the results of microwave digestion as the reference, and the effects of wet digestion on the determination of copper, iron, manganese and zinc in feeds were compared and analyzed. Results Under the optimal conditions of instrument modulation, the linearity was good for the 4 kinds of trace elements in the concentration range of 0.02?20.0 mg/L (copper, iron, manganese and zinc), and the correlation coefficients were all greater than 0.9999. The detection limits were 0.025?0.160 mg/kg, and the quantitation limits were 0.083?0.533 mg/kg. The feed samples digested by microwave digestion method and wet digestion method could obtain satisfactory recovery and repeatability, with the recovery rates of 95.7%?104.9% and relative standard deviations of less than 3%. In the actual sample measurements, comparative analysis revealed no significant differences between the microwave and wet methods for the 4 elements. Conclusion The wet digestion method can be used to replace the microwave method for sample pretreatment in daily experiments.