一种新型复合多糖提取优化和降血脂活性的研究及其胆固醇结合力预测模型的建立

Study on the extraction optimization and hypolipidemic activities of a novel compound polysaccharides and the establishment of prediction model of cholesterol-binding capacity

中文关键词:  药食同源  复合多糖  正交试验设计  数量化理论  降血脂活性  预测模型

英文关键词:medicine food homology  compound polysaccharides  orthogonal experimental design  quantitative theory  hypolipidemic activities  prediction model

中文摘要:

      目的 研究一种新型复合多糖的提取工艺、降血脂活性及其胆固醇结合力预测模型。方法 以药食同源物质山楂、荷叶、苦荞麦、决明子、枸杞以及茯苓为原料, 依照质量比4:2:2:1.5:1:1进行混合, 提取复合多糖。运用L9(34)正交试验设计和数量化理论, 优化复合多糖提取方法, 测定复合多糖的脂肪、胆固醇、胆酸盐结合力, 并构建胆固醇结合力预测模型。结果 复合多糖总糖、糖醛酸和蛋白质含量分别是76.41%、5.64%和3.18%。复合多糖胆固醇结合力最强的提取条件为: 液料比35:1 (mL/g)、提取温度65 ℃、提取时间75 min、提取方式超声辅助, 胆固醇结合力预测值是5.92 mg/g, 所建立预测方程准确可靠(R=0.95)。当样品添加量是150 mg时, 复合多糖对脂肪、胆固醇、胆酸盐结合力分别(26.49±1.68) g/g、(5.00±0.05) mg/g和(11.59±0.11) mg/g, 显著高于纤维素(P<0.05)。结论 复合多糖可作为天然脂质调节剂用于医药和功能食品领域。

英文摘要:

      Objective To study on the extraction process and hypolipidemic activities of a novel compound polysaccharides (CPs) and establish the prediction model of cholesterol-binding capacity. Methods Medicine food homology such as hawthorn, lotus leaf, Fagopyrum tataricum, semen cassiae, Lycium barbarum, and Poria cocos were taken as raw materials and mixed in a mass ratio of 4:2:2:1.5:1:1 to extract the compound polysaccharides. The L9(34) orthogonal experimental design and quantitative theory were used to optimize the extraction method of CPs, the binding capacity of fat, cholesterol and cholate of compound polysaccharides were determined, and the prediction model of cholesterol binding capacity was established. Results The content of total carbohydrate, uronic acid and protein was 76.41%, 5.64% and 3.18% in the CPs, respectively. The extraction conditions with the strongest cholesterol binding capacity of CPs were as follows: Liquid to solid ratio of 35:1 (mL/g), extraction temperature of 65 ℃, extraction time of 75 min, and ultrasonic assistance of extraction method. The predicted value of cholesterol binding capacity was 5.92 mg/g, and the established prediction equation was accurate and reliable (R=0.95). Furthermore, the binding capacity for fat, cholesterol and cholate of CPs were (26.49±1.68) g/g, (5.00±0.05) mg/g and (11.59±0.11) mg/g at a dosage of 150 mg, severally, which were significantly stronger than those of cellulose (P<0.05). Conclusion The CPs can be use as a potential natural lipid regulator in pharmaceutical and functional food field.