基于全基因组序列的污染事件中椰毒假单胞菌酵米面亚种溯源分析

Traceability analysis of Pseudomonas cocovenenans subsp. Farino fermentans in pollution events based on whole genome sequence

中文关键词:  椰毒假单胞菌酵米面亚种  湿粉及其原料米  全基因组序列  进化树  溯源

英文关键词:Pseudomonas cocovenenans subsp. Farino fermentans  wet powder and raw material rice  whole genome sequence  evolutionary tree  traceability

中文摘要:

      目的 溯源分析湿米粉与淀粉制品(统称为“湿粉”)及其原料米中分离的椰毒假单胞菌酵米面亚种。 方法 采用GB/T 4789.29—2003《食品安全国家标准 食品卫生微生物学检验 椰毒假单胞菌酵米面亚种检验》在14份湿粉及其原料米中分离出34株唐菖蒲伯克霍尔德氏菌并进行菌株全基因组重测序, 以Burkholderia_gladioli_Co14作为参比基因, 基于单核苷酸多态性(single nucleotide polymorphism, SNP)数据构建进化树, 分析不同菌株的同源关系。结果 来源于相同产地标识的样品的菌株呈现较好聚类; 来源于同一生产企业的湿粉和碎米样品的菌株具有高度同源关系。结论 提示原料米中椰毒假单胞菌酵米面亚种的基因组序列与产地溯源具有较大相关性, 在湿粉生产加工过程中存在椰毒假单胞菌酵米面亚种污染传递的风险。

英文摘要:

      Objective To trace and analyze Pseudomonas cocovenenans subsp. Farino fermentans isolated from wet rice flour, starch products(collectively referred to as "wet rice flour") and raw rice. Methods A total of 34 strains of Burkholderia gladioli were isolated from 14 portions of wet flour and its raw rice and the whole genome of the strain was re-sequenced using GB/T 4789.29—2003 National food safety standard-Microbiological examination of food hygiene-Examination of Pseudomonas cocovenenans subsp. Farino fermentans. With Burkholderia_ gladioli_Co14 as the reference gene, an evolutionary tree was constructed based on single nucleotide polymorphism data, and then the homology relationship of different strains were analyzed. Results The strains from the samples with the same origin identification showed better clustering. The strains of wet powder and broken rice samples from the same manufacturer had a high degree of homology. Conclusion It is suggested that the genome sequence of Pseudomonas cocovenenans subsp. Farino fermentans in raw rice is closely related to the origin traceability, and there is a risk of pollution transmission of Pseudomonas cocovenenans subsp. Farino fermentans in the production and processing of wet flour.