超高效液相色谱-串联三重四极杆质谱法同时检测鸡肉中7种兽药残留

Simultaneous determination of 7 kinds of veterinary drug residues in chicken by ultra performance liquid chromatography tandem triple quadrupole mass spectrometry

中文关键词:  超高效液相色谱-串联三重四极杆质谱法  兽药  鸡肉

英文关键词:ultra performance liquid chromatography tandem triple quadrupole mass spectrometry  veterinary drug  chicken

中文摘要:

      目的 建立超高效液相色谱-串联三重四极杆质谱(ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry, UPLC-MS/MS) 同时检测鸡肉中7种兽药残留的分析方法。方法 采用固相萃取提取方法, 运用Waters液相色谱柱BEH C18色谱柱进行分离, 甲醇(含0.2甲酸%)-0.2%甲酸水溶液作为流动相进行梯度洗脱, 串联质谱电喷雾正离子同时扫描, 多反应监测(multiple response monitoring, MRM)模式检测, 外标法定量。结果 该方法线性良好, 相关系数均大于0.992, 检出限为0.003~0.01 mg/kg, 平均回收率为78.8%~94.1%, 相对标准偏差(relative standard deviations, RSDs)为1.58%~5.12%。结论 该方法简便、快速、准确, 适用于鸡肉中7种兽药残留的定量分析。

英文摘要:

      Objective To establish a method for the simultaneous determination of 7 kinds of veterinary drug residues in chicken by ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry (UPLC-MS/MS). Methods Solid phase extraction and Waters liquid chromatography BEH C18 column was used for extraction and separation. Methanol (containing 0.2% formic acid)?0.2% formic acid aqueous solution was used as mobile phase for gradient elution, and tandem mass spectrometry was used for the simultaneous detection of 7 kinds of veterinary drug residues using electrospray ionization and multiple reaction monitoring (MRM) mode. External standard calibration was used for quantitative analysis. Results The linearity was good, correlation coefficients were all greater than 0.992, and the detection limit was 0.003?0.01 mg/kg. The average recoveries were 78.8%?94.1%, and the relative standard deviations (RSDs) were 1.58%?5.12%. Conclusion This method is simple, rapid, and accurate, which is suitable for quantitative analysis of 7 kinds of veterinary drug residues in chicken.