高效液相色谱法测定免疫功能型保健食品中8种功效成分

Determination of 8 kinds of functional components in immune functional health food by high performance liquid chromatography

中文关键词:  保健食品  免疫功能型  功效成分  高效液相色谱法

英文关键词:health food  immune functional type  functional composition  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法同时测定免疫功能型保健食品中8种功效成分(左旋肉碱、L-茶氨酸、天麻素、吡啶甲酸铬、咖啡因、淫羊藿苷、菊苣酸、10-羟基-α-癸烯酸)的分析方法。方法 样品用70%甲醇超声提取, 采用C18色谱柱分离, 以甲醇-磷酸二氢钾(0.1%磷酸)水溶液为流动相, 梯度洗脱, 外标法定量。结果 左旋肉碱、L-茶氨酸在10.0~150.0 mg/L, 吡啶甲酸铬、天麻素、咖啡因和淫羊藿苷在1.5~30.0 mg/L, 菊苣酸在5.0~100.0 mg/L, 10-羟基-α-癸烯酸在2.5~50.0 mg/L质量浓度与色谱峰面积有良好的线性关系, 相关系数r≥0.9990。方法检出限为1~10 mg/kg, 回收率为93.8%～108%, 相对标准偏差(relative standard deviations, RSDs)为0.7%~6.8%。结论 该方法快速、准确、灵敏, 适合保健食品中8种增强免疫力功效成分的同时测定。

英文摘要:

      Objective To establish an analytical method for the simultaneous determination of 8 functional components (L-carnitine, L-theanine, gastrodin, chromium picolinate, caffeine, icariin, cichoric acid and 10- hydroxy-α-decenoic acid) in immune functional health food by high performance liquid chromatography. Methods The samples were extracted by ultrasound with 70% methanol, and separated on a C18 column, using methanol-potassium dihydrogen phosphate (0.1% phosphoric acid) aqueous solution as the mobile phase with gradient elution, and quantified by external standard method. Results The mass concentrations of L-carnitine and L-theanine in the range of 10.0 to 150.0 mg/L, chromium picolinate, gastrodin, caffeine and icariin in the range of 1.5 to 30.0 mg/L, cichoric acid in the range of 5.0 to 100.0 mg/L, and 10-hydroxy-α-decenoic acid in the range of 2.5 to 50.0 mg/L had good linear relationship with the chromatographic peak area, and the correlation coefficient r≥0.9990. The detection limits of the method were 1?10 mg/kg, the recoveries were 93.8%?108%, and the relative standard deviations were 0.7%?6.8%. Conclusion This method is rapid, accurate and sensitive, and is suitable for the simultaneous determination of 8 functional compositions in health food.