高效液相色谱法测定草果中4种酚酸含量

Determination of 4 kinds of phenolic acids content from the Amomumtsao-ko by high performance liquid chromatography

中文关键词:  草果  高效液相色谱法  超声提取  原儿茶酸  原儿茶醛  对羟基苯甲酸  香草酸

英文关键词:Amomumtsao-ko  high performance liquid chromatography  ultrasonic-assisted extraction  protocatechuic acid  protocatechuicaldehyde  p-hydroxybenzoic acid  vanillic acid

中文摘要:

      目的 建立高效液相色谱法测定草果中4种酚酸类成分的分析方法。方法 采用高效液相色谱法, 选用C18色谱柱(250 mm×4.6 mm, 5 μm), 以甲醇和0.02 mol/L的稀磷酸溶液为流动相进行梯度洗脱, 对草果中4种主要酚类成分(原儿茶酸、原儿茶醛、对羟基苯甲酸、香草酸)的含量进行同时分析测定; 检测波长为270 nm, 流量为1.0 mL/min, 柱温为30 ℃。结果 原儿茶酸在0.04~0.36 mg (r＝0.99988)、原儿茶醛在0.04~0.36 mg (r＝0.99973), 对羟基苯甲酸在0.16~1.44 mg (r＝0.99993)、香草酸在0.04~0.36 mg (r＝0.99993)内呈良好的线性关系。平均加样回收率分别为93.3%、97.4%、97.1%、95.5%, RSD分别为2.95%、0.99%、1.34%、1.54%; 测得样品中各成分的含量范围分别为, 原儿茶酸9.12~17.42 mg/kg、原儿茶醛7.11~15.51 mg/kg、对羟基苯甲酸22.67~57.08 mg/kg、香草酸7.92~14.21 mg/kg。结论 该方法灵敏度高、稳定性强, 可较好地控制草果的质量品质。

英文摘要:

      Objective To establish a method for the determination of 4 phenolic acids from the Amomumtsao-ko by high performance liquid chromatography. Methods The content of 4 phenolic acids in Amomumtsao-ko (protocatechuic acid, protocatechualdehyde, p-hydroxybenzoic acid, vanillic acid) was analyzed and determined simultaneously by high performance liquid chromatography on a C18 column (250 mm×4.6 mm, 5 μm), using methanol and 0.02 mol/L diluted phosphoric acid as mobile phases for gradient elution. The detection wavelength was 270 nm, the flow rate was 1.0 mL/min, and the column temperature was 30 ℃. Results Protocatechuic acid, protocatechualdehyde, p-hydroxybenzoic acid, vanillic acid showed good linear relationships in the range of 0.04?0.36 mg (r=0.99988), 0.04?0.36 mg (r=0.99973), 0.16?1.44 mg (r=0.99993) and 0.04?0.36 mg (r=0.99993), respectively. The average recoveries were 93.3%, 97.4%, 97.1% and 95.5%, RSDs were 2.95%, 0.99%, 1.34% and 1.54%, respectively. The content of each index component in different samples was protocatechuic acid 9.12? 17.42 mg/kg, protocatechualdehyde 7.11?15.51 mg/kg, p-hydroxybenzoic acid 22.67?57.08 mg/kg, vanillic acid 7.92?14.21 mg/kg. Conclusion This method is highly sensitive with strong specificity and it can be used efficiently in the quality control of Amomumtsao-ko.