耐盐菌的筛选及在裙带菜加工尾水中的应用

Screening of salt-tolerant bacteria and its application in the wastewater of salted processing of Undaria pinnatifida

中文关键词:  裙带菜盐渍加工尾水  耐盐菌株筛选  化学需氧量去除率

英文关键词:waste water from Undaria pinnatifida salt processing  screening of salt tolerant strains  chemical oxygen demand removal rate

中文摘要:

      目的 筛选应用于裙带菜盐渍加工尾水处理的耐盐菌。方法 以裙带菜盐渍加工尾水为培养基, 根据CODMn去除率、甘露醇去除率以及生物量筛选出4株耐盐菌株。将这4株菌株分别应用于裙带菜盐渍加工尾水中, 以CODMn去除率为主要指标测定72 h生长曲线, 优选出各菌株的最佳培养时间。然后将这4株菌株混合应用于裙带菜盐渍加工尾水, 确定最佳接种量和培养时间。结果 经鉴定4株菌株分别为二尖梅奇酵母菌(Metschnikowiabicuspidata)、鞘氨醇菌(Sphingomonas)、胶红酵母菌(Rhodotorulamucilaginosa)、盐单胞菌(Cobetia)。各菌株对裙带菜加工尾水处理后CODMn去除率均在48 h后达到最大值, 去除率分别为84.90%、77.40%、79.20%、70.80%。4种混合菌株在28 ℃、150 r/min摇床反应条件下的最佳接种量为3%, 最佳培养时间为60 h, CODMn去除率达到83.49%。结论 本研究中筛选出的耐盐菌株有效的降低了裙带菜漂烫尾水的CODMn, 对于实际应用有重要意义。

英文摘要:

      Objective To screen the salt-tolerant bacteria used in the wastewater of salted processing of Undaria pinnatifida. Methods Using the tail water from the salted processing of Undaria pinnatifida as the medium, 4 salt-tolerant strains were screened out according to the removal rate of chemical oxygen demand (CODMn), the removal rate of mannitol and the biomass. The 4 strains were applied to the tail water of the salted wakame processing respectively, and the 72-hour growth curve was determined with the CODMn removal rate as the main indicator, and the optimal culture time of each strain was optimized. Then the 4 strains were mixed and applied to the tailwater of the salting processing of Undaria pinnatifida to determine the best inoculation amount and culture time. Results The 4 strains were identified as Metschnikowiabicuspidata, Sphingomonas, Rhodotorulamucilaginosa and Cobetia, respectively. The CODMn removal rates of all strains reached the maximum value after 48 h, and the removal rates were 84.90%, 77.40%, 79.20% and 70.80%, respectively. The optimal inoculation amount of the 4 strains was 3%, the optimal culture time was 60 h, and the CODMn removal rate reached 83.49% under the condition of 28 ℃ and 150 r/min shaking table reaction. Conclusion The salt-tolerant strains screened out in this study effectively reduce the CODMn in the tail water of the blanching of Undaria pinnatifida, which is of great significance for practical application.