海带盐渍加工尾水处理菌株筛选及生物处理工艺研究

Screening of kelp salted processing tail water treatment strains and study on biological treatment technology

中文关键词:  盐渍海带尾水  耐盐菌筛选  CODMn去除率  BOD5去除率

英文关键词:saline kelp tail water  screening of salt tolerant bacteria  chemical oxygen demand  biochemical oxygen demand

中文摘要:

      目的 筛选海带盐渍加工尾水处理菌株, 并研究其生物处理工艺。方法 将海水来源微生物通过初筛、复筛, 以甘露醇去除率为主要指标, 筛选出2株目标菌株应用于海带尾水处理, 经PCR扩增鉴定目标菌株, 以化学需氧量(chemical oxygen demand, COD)(即CODMn去除率)为指标, 通过单因素、正交实验优化2个菌株的最佳培养条件, 而后将混合菌株应用于海带盐渍加工尾水中, 研究生物处理前后海带盐渍加工尾水中CODMn去除率、生化需氧量(biochemical oxygen demand, BOD)(即BOD5去除率)的变化。结果 本研究筛选2株耐高盐的海带盐渍加工尾水处理菌株分别为二尖梅奇酵母(Metschnikowia bicuspidata)和季也蒙毕赤酵母(Meyerozyma guilliermondii), 优化条件分别为二尖梅奇酵母菌培养温度25 ℃、接种量8%和培养转速 100 r/min; 季也蒙毕赤酵母菌培养温度28 ℃、接种量5%和培养转速200 r/min; 2种菌株混合对尾水处理优化条件为接种量3%, 最佳培养时间60 h, CODMn去除率85.98%。结论 耐高盐菌株经扩繁对海带盐渍加工尾水进行了生物处理, 混合菌株生物处理降低了盐渍海带尾水中的CODMn值, CODMn去除率为81.35%; BOD5含量降低了98.70%。技术解决了褐藻加工行业高盐尾水难以处理的问题, 可为后续酵母菌处理高盐尾水提供依据。

英文摘要:

      Objective To study the strain screening and biological treatment of kelp salting processing tail water. Methods The seawater-derived microorganisms were screened and re-screened, and the removal rate of mannitol was used as the main indicator. Two target strains were screened out to be used in the treatment of kelp tail water. The target strains were identified by PCR amplification and the CODMn removal rate was used as an indicator optimized the optimal culture conditions of the two strains through single factor and orthogonal experiments, and then applied the mixed strains to the kelp salted processing tail water. Changes in the removal rate of CODMn and BOD5 in the kelp salted processing tail water before and after biological treatment were studied. Results In this experiment, two strains of salt-tolerant kelp processing tail water treatment strains were selected, namely Metschnikowia bicuspidata and Meyerozyma guilliermondii. The optimized conditions were as follows: and. Metschnikowia bicuspidata culture temperature 25 ℃, inoculation amount 8%, and culture speed 100 r/min; Meyerozyma guilliermondii culture temperature 28 ℃, inoculation amount 5% and culture speed 200 r/min. Two strains were mixed to treat tail water, and the optimized conditions were 3% inoculum, 60 h of culture time, and the removal rate of CODMn was 85.98%. Conclusion The high-salt-tolerant strains biologically treat the tail water of salted kelp after propagation. The biological treatment of mixed strains reduce the CODMn value in the tail water of salted kelp, the removal rate of CODMn is 81.35% and the content of BOD5 is reduced by 98.70%. The technology solves the problem of difficult treatment of high-salt tail water in the brown algae processing industry, and can provide a basis for subsequent yeast processing of high-salt tail water.