水热法主要提取因素对光皮木瓜多糖结构的影响

Influence of main hydrothermal extraction factors on structure of polysaccharides from Chaenomeles sinensis

中文关键词:  水热法  光皮木瓜  多糖  傅里叶变换红外光谱  X射线衍射

英文关键词:hydrothermal  Chaenomeles sinensis  polysaccharide  fourier transform infrared spectroscopy  X-ray diffraction

中文摘要:

      目的 优化水热法提取光皮木瓜(Chaenomeles sinensis)多糖的提取工艺条件, 分析光皮木瓜不溶性多糖的组成和结构。方法 光皮木瓜样品经水热法提取、抽滤、冲洗, 浓缩等操作, 分别得到不溶性多糖、醇沉多糖和醇溶多糖, 通过傅里叶变换红外光谱、扫描电子显微镜及X射线衍射分析不溶性多糖的组成和结构。结果 多糖的最佳提取条件为150 ℃/45 min, 料液比1∶10 (g/mL); 温度对水热法提取效果影响较大, 适当升高温度、延长提取时间和减少料液比有利于多糖提取; 高温虽能促进半纤维素溶解, 但无法使木质素和木聚糖降解; 不同温度提取的光皮木不溶性多糖的结构相似; 水热法提取光皮木瓜多糖不会导致多糖的功能性基团变化, 只改变多糖的化学组成。结论 该研究可为光皮木瓜多糖的结构与功能研究提供实验依据。

英文摘要:

      Objective To optimize the hydrothermal extraction conditions of Chaenomeles sinensis polysaccharides, analyze the composition and structure of insoluble polysaccharides. Methods The polysaccharides in Chaenomeles sinensis were extracted from the samples by hydrothermal, then filtered, rinsed and concentrated to obtain the insoluble polysaccharides, the alcohol-precipitated polysaccharides and the alcohol-soluble polysaccharides, respectively. The composition and the structure of the insoluble polysaccharides were analyzed by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). Results The optimum conditions for polysaccharides extracting were 150 ℃/45 min and the ratio of material to liquid was 1:10 (g/mL). Temperature had a great influence on the extraction effect of hydrothermal method. Properly increasing the temperature, extending the extraction time and reducing the material-to-liquid ratio are beneficial to the extraction of polysaccharides. Furthermore, high temperature could promote the dissolution of hemicellulose, but could not degrade lignin and xylan, and the insoluble polysaccharides extracted at different temperatures had the similar structures. The hydrothermal extraction of papaya polysaccharide did not change the functional groups of the polysaccharide, but only changed the chemical composition of the polysaccharide. Conclusion This study can provide experimental basis for the study of structure and function of Chaenomeles sinensis polysaccharide