三七药材中微生物污染的研究

Study on microbial contamination of Panax notoginseng

中文关键词:  三七  基质辅助激光解吸电离飞行时间质谱法  微生物污染

英文关键词:Panax notoginseng  matrix assisted laser desorption ionization time-of-flight mass spectrometry  microbial contamination

中文摘要:

      目的 分析和研究三七药材微生物污染情况, 探讨三七药材微生物污染的主要种类。方法 参照2015年版《中国药典》四部通则1105、1106的方法, 并结合传统生化和基质辅助激光解吸电离飞行时间质谱法(matrix assisted laser desorption ionization time-of-flight mass spectrometry, MALDI-TOF-MS)对纯化的菌落进行鉴定。最后, 运用SPSS 18.0软件对数据进行进一步地统计和分析。结果 三七药材样品在经过100 ℃、 30 min热处理后, 需氧菌总数呈现明显下降趋势, 芽孢杆菌属、水生拉恩菌为三七药材污染概率较高的菌。结论 在三七药材灭菌工艺的考察中, 应该着重杀灭芽孢杆菌, 为确保安全和药效, 需要结合多种灭菌方法。

英文摘要:

      Objective To analyze and investigate the microbial pollution of Panax notoginseng, and discuss the main types of microbial pollution of Panax notoginseng. Methods According to the method of Chinese Pharmacopoeia (2015 edition), the purified colony was identified by combining traditional biochemistry method and matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Finally, SPSS 18.0 software was used for further statistics and analysis of the data. Results After heat treatment at 100 ℃ for 30 min, the total number of aerobic bacteria in Panax notoginseng samples showed a significant downward trend, and Bacillus and Aquatic ranella were the bacteria with higher pollution probability. Conclusions In the investigation of the sterilization process of Panax notoginseng, Bacillus should be killed emphatically by combining a variety of sterilization methods, in order to ensure the safety and efficacy.