次氯酸钠消毒剂对猪黄喉中氨基脲生成量的影响

Effect of sodium hypochlorite disinfectant on the production of semicarbazone in pig aorta

中文关键词:  次氯酸钠  猪黄喉  氨基脲  浸泡条件

英文关键词:sodium hypochlorite  pig aorta  semicarbazide  soaking condition

中文摘要:

      目的 研究不同浸泡条件下次氯酸钠消毒剂对猪黄喉中氨基脲生成量的影响。方法 在不同有效氯质量浓度、浸泡时间、浸泡pH、浸泡温度下处理黄喉, 样品经甲醇+水(1:1, V:V)洗涤后在酸性条件下经邻硝基苯甲醛衍生, 再利用静电场轨道阱超高分辨质谱仪进行定性, 采用高效液相色谱质谱联用仪结合同位素内标法定量。结果 随着有效氯质量浓度(0.005%~0.2%)的增加, 猪黄喉中氨基脲生成量呈直线增加; 且随浸泡时间增加(24 h内)、pH增大、温度增高, 均会导致氨基脲含量增加。结论 经次氯酸钠消毒剂处理后, 猪黄喉中会产生一定量的氨基脲, 且产生的氨基脲以结合态的形式存在于猪黄喉中。

英文摘要:

      Objective To study the effect of sodium hypochlorite disinfectant on the production of semicarbazide in pig aorta under different soaking conditions. Methods The pig aorta was treated at different effective chlorine mass concentrations, soaking time, soaking pH, and soaking temperature. The samples were washed with methanol and water (1:1, V:V) and then derivatized with o-nitrobenzaldehyde under acidic condition. The qualitative analysis using an electrostatic field orbital trap ultra-high resolution mass spectrometer was performed, and the quantitative analysis using a high performance liquid chromatography-mass spectrometer coupled with an isotope internal standard method was performed. Results With the increase of the mass concentration of available chlorine (0.005%?0.2%), the production of semicarbazone in pig aorta increased linearly. Besides, with the increase of soaking time (within 24 h), pH and temperature, the content of semicarbazone will be increased. Conclusion After sodium hypochlorite disinfectant treatment, a certain amount of semicarbazone can be produced in pig aorta, and the semicarbazone produced exists in the form of binding state in pig aorta.