基于网络药理学探讨新疆罗勒治疗阿尔兹海默症的作用机制

Mechanism of Xinjiang Ocimum basilicum L. in the treatment of Alzheimer's disease based on network pharmacology

中文关键词:  新疆罗勒  网络药理学  阿尔兹海默症  Aβ25‒35  HT22细胞

英文关键词:Xinjiang Ocimum basilicum L.  network pharmacology  Alzheimer's disease  Aβ25‒35  HT22 cells

中文摘要:

      目的 通过网络药理学探究罗勒(Ocimum basilicum L.)活性成分其作用靶点, 并探讨其治疗阿尔兹海默症(Alzheimer's disease, AD)的作用机制。方法 按照本课题组前期研究结果, 收集罗勒的活性成分及其作用靶点, 与AD发病机制相关的靶点进行交集, 并进行蛋白-蛋白相互作用分析、基因功能和信号通路富集分析。最终, 通过以Aβ25?35诱导海马神经元细胞HT22建立阿尔兹海默病体外模型, 对罗勒活性成分进行实验验证。结果 罗勒收集槲皮素和杨梅苷等15个活性成分及其528个作用靶点, 与575个AD发病机制相关的靶点进行交集得到118个靶点。蛋白-蛋白相互作用得到31个潜在靶点, 潜在靶点参与257条基因功能与100条信号通路。结论 罗勒的活性成分可能作用于PI3K-Akt信号通路、阿尔兹海默症信号通路和TNF信号通路等, 改善细胞损伤、细胞膜的通透性以及抑制细胞凋亡, 从而达到治疗AD的目的, 体现了新疆罗勒多成分、多靶点、多通路的作用特点, 为阐述其治疗AD的作用机制提供科学依据。

英文摘要:

      Objective To explore the target of active components of Ocimum basilicum L. and its mechanism of action in the treatment of AD through network pharmacology. Methods According to the previous research results of our group, the active components and action targets of Ocimum basilicum L. were collected, and the targets related to the pathogenesis of AD were intersected, followed by protein-protein interaction analysis, gene function and signaling pathway enrichment analysis. Finally, the Alzheimer's disease model in vitro was established by inducing hippocampal neuron cell HT22 with Aβ25?3, and the experimental verification of the active ingredient in Ocimum basilicum L. was performed. Results Fifteen active components such as quercetin and myricetin and 528 action targets thereof were collected from Ocimum basilicum L., and the 118 targets were obtained through intersection with 575 targets related to the pathogenesis of AD. Thirty-one potential targets were identified through protein-protein interactions, involving 257 gene functions and 100 signaling pathways. Conclusion The active components of Ocimum basilicum L. may act on PI3K-Akt signaling pathway, Alzheimer's disease signaling pathway and TNF signaling pathway, improving cell damage, cell membrane permeability and inhibiting cell apoptosis, so as to achieve the purpose of treating AD. It reflects the multi-component, multi-target and multi-channel characteristics of Ocimum basilicum L., and provides scientific basis for expounding its mechanism of action in the treatment of AD.