国家标准方法在橄榄油α-生育酚含量测定的应用

Application of national standard method in the determination of alpha tocopherol in olive oil

中文关键词:  α-生育酚  橄榄油  高效液相色谱法

英文关键词:α-tocopherol  olive oils  high performance liquid chromatography

中文摘要:

      目的 探究反相高效液相色谱法和正相高效液相色谱法检测橄榄油α－生育酚含量。方法 反相高效液相色谱法采用C30柱, 流动相甲醇/水梯度洗脱,流速为0.8 mL/min, 柱温为20 ℃, 紫外检测波长为 294 nm; 正相高效液相色谱法采用Si120硅胶色谱柱, 流动相为正己烷/1,4二氧六环按(95:5), 流速为 0.8 mL/min, 柱温为30 ℃, 荧光检测激发波长为294 nm, 发射波长为328 nm。结果 反相色谱法测定α-生育酚的线性范围为2.00~60.00 μg/mL, 定量限为0.6 μg/mL, 精密度(relative standard deviation, RSD)为5.5, 回收率为90%~106%, RSD为5.5%。正相色谱法测定α-生育酚的线性范围0.200~6.00 μg/mL, 定量限为0.014 μg/mL, 精密度RSD为1.8%, 回收率为99%~104%, RSD为1.2%。结论 正相色谱简便、更快速、更灵敏、更准确, 更适合初榨橄榄油中α-生育酚的定量测定。

英文摘要:

      Objective To investigate the determination of α-tocopherol content in olive oil by reversed phase-high-performance liquid chromatography (RP-HPLC) and normal phase-high-performance liquid chromatography (NP-HPLC). Methods RP-HPLC was performed on a C30 column with a gradient elution of methanol/water as mobile phase at a flow rate of 0.8 mL/min. The column temperature was set at 20 ℃, and the UV detection wavelength was set at 294 nm. The NP-HPLC was performed on a Si120 silica gel column with n-hexane/1, 4-dioxane (95:5) as mobile phase at a flow rate of 0.8 mL/min. The column temperature was set at 30 ℃. The excitation wavelength and emission wavelength for fluorescence detection were 294 nm and 328 nm. Results The linear range of α-tocopherol was 2.00?60.00 μg/mL, the limit of quantification was 0.6 μg/mL, the precision RSD was 5.5, the recoveries were 90%~105%, and the RSD was 5.5%. The linear range of α-tocopherol was 0.200? 6.00 μg/mL, the limit of quantification was 0.014 μg/mL, the precision RSD was 1.8%, the recoveries were 99%?103%, and the RSD was 1.2%. Conclusion RP-HPLC is simple, faster, more sensitive and more accurate. It is more suitable for the quantitative determination of α-tocopherol in virgin olive oil.