固相萃取-气相色谱串联质谱法测定梅州地区客家娘酒中的氨基甲酸乙酯及其食用风险评估

Determination of ethyl carbamate in Hakkaniang wine in Meizhou region by solid phase extraction-gas chromatography tandem mass spectrometry and its edible risk assessment

中文关键词:  客家娘酒  鸡子酒  氨基甲酸乙酯  风险监测  风险评估

英文关键词:Hakka wine  “Jizi” wine  ethyl carbamate  risk monitoring  risk assessment

中文摘要:

      目的 建立固相萃取-气相色谱串联质谱法测定客家娘酒中氨基甲酸乙酯含量的分析方法, 并对梅州地区客家娘酒进行食用风险评估。方法 客家娘酒中的氨基甲酸乙酯(ethyl carbamate, EC)通过乙酸乙酯提取后, 提取液经N-丙基乙二胺进行基质固相萃取净化, 采用气相色谱-串联质谱法(gas chromatography-mass/mass spectrometry, GC-MS/MS)进行测定, 内标法定量。结果 该方法回收率95.01%~99.88%, 相关系数r2为0.9999, 检出限1.1 μg/kg, 定量限3.7 μg/kg。客家娘酒中EC的含量在52.28~433.82 μg/kg之间, 平均值为157.12 μg/kg。高温烹煮会使客家娘酒中的EC含量上升, 在与鸡肉同煮的情况下, 酒液中EC减少约40%。结论 该方法可以满足客家娘酒中EC的检测需求。通过风险评估发现, 从18岁以上全部人群平均水平来看, 客家娘酒中EC的风险较低, 但饮酒者和产褥期妇女的暴露限值(margin of exposure, MOE)分别为450和143, 存在较大的公共卫生风险。

英文摘要:

      Objective To establish a method for the determination of ethyl carcarate in Hakka wine by solid phase extraction-gas chromatography-tandem mass spectrometry, and evaluate the edible risk of Hakka wine in Meizhou area. Methods The ethyl carbamate (EC) in Hakka wine was extracted by ethyl acetate, and the extract was purified by matrix solid phase extraction (SPE) with N-propylenediamine, determined by gas chromatography-mass/mass spectrometry (GC-MS/MS) and quantified by internal standard method. Results The recovery rates of this method were 95.01%?99.88%, the correlation coefficient r2 was 0.9999, and the limit of detection was 1.1 μg/kg, the limit of quantitation was 3.7 μg/kg. The content of EC in Hakka liquor was between 52.28 and 433.82 μg/kg, with an average value of 157.12 μg/kg. The EC content in Hakka wine would increase in high-temperature cooking. When cooked with chicken, the EC content in the wine would be reduced by about 40%. Conclusion This method can meet the detection requirements of EC in Hakka wine. The risk assessment finds that from the average level of all people over the age of 18, the risk of EC in Hakka wine is low, but the margin of exposure (MOE) for drinkers and puerperium women are 450 and 143, respectively, and there is a greater public health risk.