食品检测能力验证中沙门氏菌的分离鉴定

Isolation and identification of Salmonella proficiency testing in food testing

中文关键词:  沙门氏菌  鉴定  能力验证  实时荧光环介导等温扩增法

英文关键词:Salmonella  identification  proficiency testing  real-time fluorescence loop-mediated isothermal amplification method

中文摘要:

      目的 参加编号为CFAPA-375的食品中沙门氏菌的检测能力验证, 验证实验室沙门氏菌的检测能力。方法 采用国家标准法检测沙门氏菌能力验证样品, 同时采用实时荧光环介导等温扩增(loop-mediated isothermal amplification, LAMP)法作为辅助方法。对分离出的可疑菌落, 采用全自动微生物鉴定系统和实时荧光LAMP法进行全面鉴定。结果 样品N1和N3中检出沙门氏菌, 样品N2中未检出沙门氏菌, 实时荧光LAMP法与国家标准法检测结果一致。结论 本次实验室的能力验证结果为“满意”, 验证了实验室沙门氏菌的检测能力。实时荧光LAMP法快速、简便、特异性强, 可与国家标准法配合使用, 以确保实验结果的准确快速。

英文摘要:

      Objective To participate in proficiency testing of Salmonella in food numbered CFAPA-375, and verify the testing ability of Salmonella in laboratory. Methods The national standard method was used to detect the samples of Salmonella proficiency testing, and the real-time fluorescence loop-mediated isothermal amplification (LAMP) method was used as an auxiliary method. The suspicious colonies were identified by automatic microbial identification system and real-time fluorescence LAMP method. Results Salmonella was detected in sample N1 and N3, while was not detected in sample N2. The results of real-time fluorescence LAMP method were consistent with the national standard method. Conclusion The proficiency testing results of the laboratory are satisfactory, which effectively verifies the laboratory's ability to detect Salmonella. Real-time fluorescence LAMP method is fast, simple and specific, which can be used in conjunction with national standard method to ensure the accuracy and speed of experimental results.