高效液相色谱-串联质谱法检测减肥茶中的匹可硫酸钠

Determination of sodium picosulfate in slimming tea by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  高效液相色谱-串联质谱法  匹可硫酸钠  减肥茶

英文关键词:high performance liquid chromatography-tandem mass spectrometry  sodium picosulfate  slimming tea

中文摘要:

      目的 建立高效液相色谱-串联质谱法检测减肥茶中非法添加匹可硫酸钠的分析方法。方法 样品经1 mol/L盐酸溶液提取后, 经过聚酰胺固相萃取小柱净化, 采用Agilent RRHD Eclipse Plus C18柱(2.1 mm× 50 mm, 1.8 μm)分离, 以乙腈-乙酸铵溶液为流动相梯度洗脱, 多反应监测模式下检测, 外标法定量。结果 匹可硫酸钠在5~500 ng/mL时响应值与浓度呈良好线性关系, 方法检出限为0.125 mg/kg, 样品的加标回收率为90.4%~99.1%, 相对标准偏差为2.8%~6.9%。结论 本方法通过对目标物的有效净化和富集, 能有效除去基质干扰、提高方法灵敏度、降低基质效应、实现快速测定、准确定量和确证的目的, 适用于减肥茶中匹可硫酸钠的检测。

英文摘要:

      Objective To establish an analytical method for the illegal addition of sodium picosulfate in diet tea by high performance liquid chromatography- tandem mass spectrometry. Methods The samples were extracted with 1 mol/L hydrochloric acid solution, purified with polyamide solid phase extraction column, and separated by Agilent RRHD Eclipse Plus C18 column (2.1 mm×50 mm, 1.8 μm). The mobile phase was gradient elution with acetonitrile-ammonium acetate solution. The detection was carried out in multi-reaction monitoring mode, and the quantitative analysis was carried out by external standard method. Results The detection limit of the method was 0.125 mg/kg. The recoveries of the samples were 90.4%?99.1%, and the relative standard deviations were 2.8%?6.9%. Conclusion The method can effectively remove the matrix interference, improve the sensitivity of the method, reduce the matrix effect, and achieve the purpose of rapid determination, accurate quantitative and confirmatory through the effective purification and enrichment of the target substance. It is suitable for the determination of sodium picosulfate in diet tea.