基于响应面法优化杜仲雄花茶中活性成分及加工工艺

Optimization of the active ingredients and processing technology of Eucommia ulmoides male flower tea based on response surface methodology

中文关键词:  杜仲雄花  响应面法  加工工艺参数  桃叶珊瑚苷  京尼平苷酸

英文关键词:Eucommia ulmoides male flower  response surface methodology  processing parameters  aucubin acid  geniposidic acid

中文摘要:

      目的 采用响应面法探究杜仲雄花茶中含量较高的环烯醚萜类活性成分。方法 采用液相色谱法对以杜仲雄花为原料加工而成的杜仲雄花茶中7种环烯醚萜类化合物进行检测分析, 利用其中含量较高的桃叶珊瑚苷和京尼平苷酸为指标, 在单因素实验结果的基础上通过Box-Behnken实验设计, 优化杀青温度、杀青时间、揉捻时间等加工工艺参数。结果 在杀青温度250 ℃、杀青时间2 min、揉捻时间2 min的工艺条件下, 桃叶珊瑚苷和京尼平苷酸的含量达到了8.99 mg/g。结论 采用响应面法优化杜仲雄花茶工艺合理可行。

英文摘要:

      Objective To investigate the high content of iridoids in Eucommia ulmoides male flower tea by response surface methodology. Methods Seven iridoids in Eucommia ulmoides male flower tea were detected and analyzed by high performance liquid chromatography. The processing parameters, such as temperature, time and rolling time, were optimized by Box-Behnken experiment design based on single factor experimental results, using the higher content of aucubin and geniposidic acid as indexes. Results The content of aucubin and geniposidic acid reached 8.99 mg/g when fixing temperature was 250 ℃, fixing time and kneading time were both 2 min. Conclusion It is reasonable and feasible to optimize the process of Eucommia ulmoides male flower tea by response surface methodology