鲟鱼软骨II型胶原蛋白的提取工艺优化

Optimization of extraction process of collagen type II from sturgeon cartilage

中文关键词:  鲟鱼软骨  II型胶原蛋白  酶解  水解度  粘多糖

英文关键词:sturgeon cartilage  collagen type II  enzymatic hydrolysis  degree of hydrolysis  mucopolysaccharide

中文摘要:

      目的 以鲟鱼软骨为原料, 提取II型胶原蛋白并优化其工艺参数。方法 选热解-酶解法提取制备II型胶原蛋白, 并对工艺中影响提取效果的各个因素进行单因素试验, 最后通过正交试验获得最优参数。结果 软骨提取最佳工艺参数: 热解温度120 ℃处理30 min, 加酶量8000 U/g?Pro, 酶解时间4 h, 酶解pH 8, 酶解温度50 ℃。采用上述最优参数提取鲟鱼软骨中II型胶原蛋白的水解度为10.85%, 蛋白质含量70.7%, 对该提取物检测分析粘多糖-硫酸软骨素含量, 可得其含量为30.6%(以干基计)。结论 该提取工艺重复性好, 适用于鲟鱼软骨II型胶原蛋白的提取, 为下一步其活性功能研究提供理论数据基础。

英文摘要:

      Objective To extract collagen type II from sturgeon cartilage using sturgeon cartilage as raw material and optimize its technical conditions. Methods The collagen type II was isolated through a method of pyrolysis-enzyme hydrolysis, and single factor test orthogonal experiment was used to optimize the extraction process of collagen. Results The best extraction technology of sturgeon cartilage was: the pyrolysis 120 ℃ of 30 min, enzyme dosage of 8000 U/g pro, hydrolysis time 4 h, enzymolysis pH 8, the temperature of 50 ℃. The degree of hydrolysis of the collagen type II extracted from sturgeon cartilage was 10.85%, the content of protein was 70.7%, and the content of mucopolysaccharide chondroitin sulfate was 30.6% (dried). Conclusion This method has good repeatability, it can be used for extraction of type II collagen from sturgeon cartilage, and provides theoretical data basis for the research on bioactivity and function.