婴幼儿配方乳粉中维生素B1前处理方法的优化及评估

Optimization and evaluation of the pretreatment conditions of vitamin B1 in infant formula milk powder

中文关键词:  维生素B1  婴幼儿配方乳粉  水解  酶解

英文关键词:vitamin B1  infant formula milk powder  hydrolysis  enzymatic hydrolysis

中文摘要:

      目的 优化维生素B1测定中酸水解过程, 评估酶解过程所用酶的种类。方法 对样品分别用高压灭菌和沸水浴2种方法进行水解, 比较2种方法测得的维生素B1含量; 并在酶解时对加入酶的种类进行考察, 评估对检测结果的影响。结果 2种水解方法的测定结果无显著性差异, 方法回收率为86.0%~94.6%; 加入酸性磷酸酶会导致维生素B1含量明显降低。结论 沸水浴法可用于维生素B1的含量测定, 方法快速、可批量检测。酶解过程中酸性磷酸酶的加入对实验结果影响的评估也对日后进行多种维生素检测方法的整合提供了技术参考。

英文摘要:

      Objective To optimize the acid hydrolysis process in vitamin B1 determination and to evaluate the type of enzyme used in the enzymatic hydrolysis process. Methods The samples were hydrolyzed by high pressure sterilization and boiling water bath respectively and the content of vitamin B1 measured by the two methods was compared. During the enzymatic hydrolysis, the types of enzymes added were investigated and the influence on the detection results was evaluated. Results There was no significant difference between the results of the two hydrolysis methods, and the method recovery rate was 86.0%–94.6%. Theaddition of acid phosphatase resultedin a significant decrease in vitamin B1 content. Conclusion The boiling water bath method can be used for the determination of vitamin B1 content, and the method is rapid and can be used for batch detection. The evaluation of the effect of acid phosphatase addition on the experimental results during the enzymatic hydrolysis also provided a technical reference for the integration of multi-vitamin detection methods in the future.