三七茎叶总皂苷提取工艺及抗氧化活性研究

Study on the extraction technology and antioxidant activity of total saponins from stem-leaf of Panax notoginseng

中文关键词:  七茎叶  总皂苷  加热回流  响应面法  抗氧化活性

英文关键词:Panax notoginseng stem-leaf  total saponins  heating reflux extraction technology  response surface methodology  antioxidant activity

中文摘要:

      目的 建立并优化了乙醇热回流提取三七茎叶总皂苷的工艺条件, 高效提取三七茎叶总皂苷。方法 以人参皂苷Rb3的提取率和纯度为指标, 通过部分析因设计和中心组合设计实验, 对乙醇浓度、液料比、提取时间、提取温度4个因素进行考察, 筛选最佳提取工艺参数, 并进一步对4种不同提取方法所获三七茎叶总皂苷的抗氧化活性进行评价。结果 最佳提取工艺参数为乙醇浓度50%、液料比10 (mL/g)、提取时间1 h和提取温度60 ℃, Rb3提取率为90.6%±1.77%, 纯度为8.41%±0.39%, 且该提取方法所获皂苷的抗氧化活性高于其他提取方法。结论 该工艺流程在保证获得较高人参皂苷Rb3纯度和含量的基础上, 具有经济、快速、高效的特点, 为后续三七茎叶的进一步开发奠定了理论基础。

英文摘要:

      Objective To establish and optimize the process conditions for extracting total saponins from stem-leaf of Panax notoginseng with the heating reflux extraction technology, and extract the total saponins from Panax notoginseng stems and leaves efficiently. Methods With the extraction rate and purity of ginsenoside Rb3 as the index, 4 factors including ethanol concentration, liquid-solid ratio, extraction time and extraction temperature were investigated through partial factor experiment design and central composite experiment design to select the best extraction process parameters. The antioxidant activity of total saponins from stem-leaf of Panax notoginseng obtained by 4 kinds of different extraction methods was evaluated further. Results The optimum extraction conditions were as follows: Ethanol concentration was 50%, liquid-solid ratio was 10 (mL/g), extraction time was 1 h and extraction temperature was 60 ℃. The extraction rate of Rb3 was 90.6%±1.77%, and the purity was 8.41%±0.39%. The antioxidant activity of total saponins from stem-leaf of Panax notoginseng obtained by this method was higher than that of other extraction methods. Conclusion On the basis of ensuring high purity and content of ginsenoside Rb3, the process is economic, rapid and efficient, which lays a theoretical foundation for further development of Panax notoginseng stem-leaf.