超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法测定动源性食品中33种兽药残留及质谱库的建立

Detection of veterinary drug residues in animal-derived foods by ultra performance liquid chromatography-high resolution mass spectrometry and establishment of mass library

中文关键词:  超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法  兽药残留  动源性食品  质谱库

英文关键词:ultra performance liquid chromatography-high resolution mass spectrometry  veterinary drug residues  animal-derived foods  mass library

中文摘要:

      目的 建立超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法测定动源性食品中33种兽药残留的检测方法。方法 采用乙腈-水(80:20, V:V)为提取溶剂, QuEChERS方法净化, 在Full MS-ddMS2正离子扫描模式下, 快速对动源性食品中的目标化合物进行检测。结果 在r=70000, 扫描范围80~1000 m/z内, 33种目标化合物的线性相关系数均大于0.99, 基质加标回收率范围为70.4%~97.3%, 相对标准偏差(relative standard deviation, RSD)为2.1%~6.0%(n=6)。结论 建立的方法适用于动源性食品中33种兽药残留快速检测。同时, 本研究建立的33种兽药高分辨质谱库, 可应用于非目标化合物的定性筛查。

英文摘要:

      Objective To establish a method for the determination of 33 veterinary drug residues in animal -derived foods by ultra performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) . Methods The samples were extract with acetonitrile-water (80:20, V:V), and purified by QuEChERS. In the Full MS ddMS2 positive ion scanning mode, the target compounds in animal-derived foods food were detected quickly. Results Under the conditions of Full MS(80~1000 m/z) and 70000 mass resolution, the 33 veterinary drug residues had good linear relationships (r≥0.99). The matrix recoveries were between 70.4%?97.3%. The relative standard deviations were between 2.1%?6.0% (n=6). Conclusion The established method is suitable for rapid detection and analysis of 33 veterinary drug residues in animal-derived foods. An accurate mass library has been established and the library can be used to screen and confirm the non-target compounds.