3种快速检测柳州螺蛳粉中热损伤沙门氏菌方法的比较分析

Comparative analysis of 3 rapid detection methods for thermal injury Salmonella in Liuzhou snail of rice noodle

中文关键词:  热损伤  沙门氏菌  柳州螺蛳粉  快速检测

英文关键词:thermal injury  Salmonella  Liuzhou snail of rice noodle  rapid detection

中文摘要:

      目的 比较实时荧光PCR法、全自动免疫分析仪法(vitek immune diagnostic assay system, VIDAS)和环介导等温扩增法(loop-mediatedisothermalamplification, LAMP)检测柳州螺蛳粉中热损伤沙门氏菌。方法 以预包装柳州螺蛳粉为样品, 利用3种热损伤沙门氏菌以人工添加的方式模拟污染样品, 污染水平为4 CFU/ 25 g和12 CFU/25 g, 通过一步增菌和二步增菌后分别用实时荧光PCR、VIDAS和LAMP检测, 并与国家标准法比较。结果 当3种快速检测方法对只经过一步增菌的3种热损伤沙门氏菌进行检测时, 7组VIDAS检测结果和2组LAMP检测结果出现假阴性; 对40份模拟样品检测结果显示, VIDAS无法检测柳州螺蛳粉中低污染水平的热损伤亚利桑那沙门氏菌。结论 实时荧光PCR法相对VIDAS法和环介导等温扩增法更适合基质复杂且沙门氏菌污染水平低样品的快速检测。

英文摘要:

      Objective To compare the detection of thermal injured Salmonella in Liuzhou snail of rice noodle by the method of real-time PCR, the automated immunoassay analyzer (VIDAS) and loop-mediated isothermal amplification (LAMP), respectively. Methods Three thermal injured Salmonella strains were added to the sample of Liuzhou snail of rice noodle artificially, the addition was 4 CFU/25 g and 12 CFU/25g. Compared with national standard method, the samples were detected by the method of real-time PCR, the automated VIDAS and LAMP with one-step and two-step bacterial proliferation, respectively. Results When three rapid detection methods were used to detect three types of heat-damaged Salmonella only after one-step proliferation, the results of 7 groups of VIDAS and 2 groups of LAMP showed false negative results. Tests on 40 simulated samples showed that VIDAS was unable to detect the low contamination levels of heat-damaged Salmonella Arizona in Liuzhou snail rice noodle.Conclusion The real-time PCR is more effective in rapidly detecting the sample contained low level of Salmonella with complicate base than VIDAS and LAMP.