G-四链体特性及其基于核酸辅助检测方法的研究进展

Review of characteristics of G-quadruplex and development of its nucleic acid assisted detection method

中文关键词:  G-四链体  脱氧核酶  核酸改造  核酸扩增  检测技术

英文关键词:G-quadruplex  DNAzyme  nucleic acid modification  nucleic acid amplification  detection techniques

中文摘要:

      G-四链体(G-quadruplex)是多聚鸟嘌呤(G)重复序列通过Hoogsteen氢键配对形成的由四链构成的非典型二级结构, 可与氯化血红素(hemin)结合, 形成具有类似过氧化物酶活性的脱氧核酶, 可使其底物显色, 或改变某些染料的荧光强度, 起到生物传感器的作用。G-四链体作为功能性核酸, 以其稳定、灵敏、特异性强等优势, 受到越来越多的重视。本文主要综述了G-四链体的脱氧核酶特征、G-四链体的改造和修饰、G-四链体结构的诱导形成、其与核酸分析技术联用构建的多种检测方法及在检测方面应用的研究进展和趋势。

英文摘要:

      A G-quadruplex is a noncanonicalsecondarystructure composed of four strands formed by repeating sequences of polyguanine (G) through Hoogsteen hydrogen bonding. It can combine with hemin to form a deoxyribozyme (DNAzyme) with similar peroxidase activity, which can make its substrate changecolor, or alter the fluorescence intensity of certain dyes to play the role of a biosensor. As a functional nucleic acid, G-quadruplex has received more and more attention because of its stability, sensitivity, and strong specificity. This paper mainly reviewedthe characteristics of G-quadruplexas DNAzyme, the modification of G-quadruplex, the induced formation of G-quadruplex structure, its multiple detection methods established in combination with nucleic acid analysis technology as well as the research progress and trends in the application of detection methods.