响应面法优化海参蒸煮废液皂苷的提取工艺

Optimization of extraction process of saponins from sea cucumber cooking waste liquid by response surface method

中文关键词:  海参  蒸煮废液  海参皂苷  响应面分析法

英文关键词:sea cucumber  cooking waste liquid  sea cucumber saponins  response surface analysis

中文摘要:

      目的 优化海参蒸煮废液中皂苷的提取工艺。方法 通过单因素试验, 研究了乙醇体积分数、提取温度、料液比和提取时间4个因素对皂苷提取率的影响, 并通过Box-Benhnken中心组合试验及响应面分析法对提取条件进行优化。结果 最优提取工艺条件为: 乙醇体积分数64%, 提取温度56 ℃, 料液比1:2 g/mL, 海参皂苷的提取率为1.68%。结论 经过工艺优化, 海参皂苷的提取率明显提高, 为海参废液的资源化利用提供依据。

英文摘要:

      Objective To optimize the extraction process of saponins from sea cucumber cooking waste liquid. Methods Through single factor experiment, the influence of ethanol volume fraction, extraction temperature, rate of material and solution and extraction time on the extraction yield of saponin was studied, and the extraction conditions were optimized by Box-Benhnken central combination experiment and response surface analysis. Results The optimal extraction conditions were 64% of ethanol volume fraction, extraction temperature 56 ℃, rate of material and solution 1:2 g/mL. Under these conditions, the degree of extraction yield was 1.68%. Conclusion After optimization of the processes, the extraction rate of sea cucumber saponins is significantly improved, which provides the basis for the resource utilization of sea cucumber waste liquid.