酸浆中木犀草苷提取工艺优化

Optimization of extraction process of luteoloside from Physalis alkekengi L. var. franchetii (Mast.) Makino

中文关键词:  酸浆  木犀草苷  加热回流提取  正交实验

英文关键词:Physalisalkekengi L. var. franchetii (Mast.) Makino  luteoloside  heating reflux extraction  orthogonal experiment

中文摘要:

      目的 优化酸浆中木犀草苷成分的提取工艺。方法 选取干燥的酸浆[Physalisalkekengi L. var. franchetii (Mast.) Makino]宿萼作为实验材料, 分别使用甲醇、乙醇和水作为溶剂, 考察超声、热回流和渗漉对木犀草苷提取效率的影响。结果 以乙醇作为提取溶剂, 采用加热回流的提取方式, 木犀草苷得率较高。运用单因素考察法及正交实验确定了最佳工艺条件: 使用乙醇溶液, 料液比1:30(g/mL), 加热回流90 min, 提取3次, 木犀草苷得率可达1.28%。结论 经过优化后, 酸浆提取所选用试剂安全可靠, 木犀草苷得率显著提高。

英文摘要:

      Objective To optimize the extraction process of luteoloside from Physalisalkekengi L. var. franchetii (Mast.) Makino. Methods For extraction of luteoloside from the dried calyxes of Physalisalkekengi L. var. franchetii (Mast.) Makino, the solvents of methanol, ethanol and water, and the methods of ultrasonic, heating reflux extraction and percolation were investigated, respectively. Results The yield of luteoloside was higher by using the solvent of ethanol and the method of heating reflux. Then, single factor experiment and orthogonal experiment were applied for further optimization of the extraction process. The results showed that the yield of luteoloside was increased to 1.28% by using the solvent of ethanol, the ratio of material to solvent 1:30 (g/mL), heating reflux extraction for 90 min, extraction of 3 times. Conclusion After optimization of the processes, the extraction rate of luteoloside is significantly improved.