食荚豌豆和豌豆中螺虫乙酯及其代谢物残留和膳食摄入风险评估

Risk assessment of spiromethyl and its metabolites residues and dietary intake in edible podded and peas

中文关键词:  螺虫乙酯  主要代谢产物  膳食摄入评估  食荚豌豆  豌豆  高效液相色谱-三重四极杆串联质谱法

英文关键词:spirotetramat  main metabolites  risk assessment for dietary residue intake  edible podded  peas  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 研究食荚豌豆和豌豆中螺虫乙酯主要代谢物的转化情况, 施药措施与残留量的相关性, 残留量膳食摄入评估。方法 样品中螺虫乙酯及其代谢物加乙腈提取, N-丙基乙二胺和石墨化碳黑净化, 离心, 上清液过滤膜后高效液相色谱-三重四极杆串联质谱法检测, 对施用过螺虫乙酯的食荚豌豆和豌豆样品进行检测分析及膳食摄入评估。结果 食荚豌豆和豌豆中螺虫乙酯及其4个主要代谢物的平均回收率分别为82%~113%和84%~108%, 相对标准偏差分别为1.0%~11.3%和1.7%~19%, 最低检测浓度均为0.01 mg/kg。食荚豌豆中螺虫乙酯风险评估定义残留量为0.05~0.58 mg/kg, 监测定义残留量为0.02~0.55 mg/kg; 豌豆中螺虫乙酯风险评估定义残留量为0.05~0.11 mg/kg, 监测定义残留量为0.02~0.090 mg/kg。食荚豌豆中主要代谢物是酮基-羟基和烯醇, 豌豆中是烯醇。螺虫乙酯及其代谢物在食荚豌豆中的残留量与施药次数和施药剂量呈正相关, 与采收间隔期呈负相关。螺虫乙酯普通人群的国家估计每日摄入量是0.191 mg, 占日允许摄入量的6.07%左右。结论 按本研究的施药剂量、施药次数和采收间隔期进行施药, 对一般人群健康不会产生不可接受的风险。

英文摘要:

      Objective To study the transformation of spirotetramat main metabolites in edible podded and peas, relation between application measures and residues, risk assessment for dietary residue intake. Methods The residue of spirotetramat and its main metabolites were extracted with acetonitrile, primary secondary amine and graphitized carbon black purification, centrifugated, the supernatant was filtered through the membranes, and analyzed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The dietary residue intake of spirotetramat and its metabolites in edible podded and peas were analyzed and evaluated. Results The average recoveries of spirotetramat and its metabolites in edible podded and peas were 82%?113%, and 84%?108%, with a relative standard deviation (RSD) of 1.0%?11.3%, and 1.7%?19%, respectively. The limits of quantification (LOQ) were 0.01 mg/kg for spirotetramat and its metabolites in edible podded and peas. Residue for dietary intake and enforcement in edible podded of spirotetramat were 0.05?0.58 mg/kg and 0.02?0.55 mg/kg, respectively. Residue for dietary intake and enforcement in peas of spirotetramat were 0.05?0.11 mg/kg and 0.02?0.090 mg/kg, respectively. The main metabolites in edible podded were ketohydroxy and enol, in peas were enol. The residues of spirotetramat and its metabolites in edible podded were positively correlated with the number of times sprayed and dosages, but negatively correlated with the days after spraying. The national estimated daily intake of spironolactone for the general population was 0.191 mg, and accounted for about 6.07% of the daily allowable intake. Conclusion According to the dose, frequency and harvest interval of this study, there will be no unacceptable risk to the health of the general population.