河北省小麦、玉米及其制品中16种真菌毒素污染水平调查与分析

Investigation and analysis of 16 kinds of mycotoxins pollution levels in wheat, corn and products in Hebei province

中文关键词:  河北  小麦  玉米  真菌毒素

英文关键词:Hebei  wheat  corn  mycotoxins

中文摘要:

      目的 调查分析河北省小麦、玉米及其制品中16种常见真菌毒素污染状况。方法 收集河北省37份鲜(冻)玉米/玉米粒、74份玉米、玉米面制品以及240份小麦样品。样品经乙腈:水:甲酸(79:20:1, V:V:V)溶液超声离心提取后, 通过同位素内标稀释法处理, 采用液相色谱-串联质谱法进行检测。结果 伏马毒素小麦检出8份, 玉米、玉米面制品检出66份, 检出范围为ND~3889.9 μg/kg; 玉米赤霉烯酮检出小麦检出2份, 玉米、玉米面制品检出69份, 检出范围为ND~323.2 μg/kg; 脱氧雪腐镰刀菌烯醇小麦检出122份, 玉米、玉米面制品检出68份, 检出范围为ND~4183.4 μg/kg; 3-乙酰基-脱氧雪腐镰刀菌烯醇小麦检出5份, 玉米、玉米面制品检出18份; 15-乙酰基-脱氧雪腐镰刀菌烯醇小麦检出12份, 玉米、玉米面制品检出38份; 其他毒素均为未检出。鲜(冻)玉米/玉米粒中16种真菌毒素均未检出。结论 伏马毒素、玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇为小麦、玉米及其制品的主要污染物, 其污染状况应引起关注。

英文摘要:

      Objective To investigate the contamination situation of 16 common mycotoxins in wheat, corn and their products from Hebei province. Methods Totally 37 fresh (frozen) corn/corn kernels, 74 corn and corn flour products and 240 wheat samples were collected. The samples were extracted by ultrasonic centrifugation of acetonitrile: water: formic acid (79:20:1, V:V:V) solution, then treated by isotope internal standard dilution method, and detected by liquid chromatography tandem mass spectrometry. Results 8 samples of fumonisins were detected in wheat, 66 samples were detected in corn and corn flour products, and the detection range was ND~3889.9 μg/kg; 2 samples were detected in wheat zearalenone, and 69 samples were detected in corn and corn flour products. The detection range is ND~323.2 μg/kg; 122 samples of deoxynivalenol wheat were detected, 68 samples of corn and corn flour products were detected, and the detection range was ND~4183.4 μg/kg; 5 samples of 3-acetyl-deoxynivalenolwere detected in wheat, 18 samples were detected in corn and corn flour products; 12 samples of 15-acetyl-deoxynivalenolwere detected in wheat, 38 samples were detected in corn and corn flour products; others Toxins were not detected. Conclusion Fumonisins, zearalenone and deoxynivalenol are the main pollutants of wheat, corn and their products, and their pollution status should be paid attention to.