侧向层析免疫试纸法快速检测蔬菜中的腐霉利

Rapid detection of saprophyte in vegetables by lateral chromatography immunoassay

中文关键词:  侧向层析试纸  快速检测  蔬菜  腐霉利

英文关键词:lateral flow test strip  rapid detection  vegetables  procymidone

中文摘要:

      目的 建立基于胶体金标记的侧向层析免疫试纸快速检测新技术检测多种蔬菜中腐霉利的分析方法。方法 利用喷膜仪以0.5 μL/cm的喷速将1 mg/mL的羊抗鼠抗体、1 mg/mL的腐霉利包被原喷到NC膜上。再通过静电吸附的方式将腐霉利单克隆抗体标记到胶体金表面, 制备成偶联物滴加到标记垫上。最后依次将样品垫, 标记垫、划好的NC膜以及吸水垫粘贴到底板上, 两者之间均有2 mm的重叠, 使标记垫处于样品垫之下、NC膜之上的位置, 组装好完整的试纸条, 测试过程中在样品垫上滴加样品, 观察C、T线位置显线情况进行判定。结果 该方法不受蔬菜中本身复杂成分以及背景颜色的影响, 能够对蔬菜中腐霉利进行特异性识别鉴定, 10 min便可获得检测结果。结论 该方法测试的结果与仪器法检测结果一致, 检测灵敏度满足国标限量要求。

英文摘要:

      Objective To establish a method for detection the procymidone in various vegetables by rapid detection method of lateral chromatography immunoassay based on colloidal gold labeling. Methods Goat anti-mouse antibody and procymidon-BSA (1 mg/mL) were sprayed onto the NC membrane at a spray rate of 0.5 μL/cm by spray membranes instrument, then the monoclonal antibody was labeled to the colloidal gold surface by electrostatic adsorption, and the conjugated compound was prepared and added to the labeling pad. Finally, the sample pad, marking pad, NC film and absorbent pad were pasted to the bottom plate, there was a 2 mm overlap between the two, so that the conjugation pad was located under the sample pad and above the NC film, then, complete test strip was assembled. During the test, the sample was added to the sample pad, and the position of C and T lines were observed for determination. Results This method was independent of the complex components and background color in vegetables, and could specifically identify bean curd in vegetables, and the detection results could be obtained within 10 min. Conclusion The test result of this method is consistent with that of instrument method, and the detection sensitivity meets the requirement of national standard limit.