湖南茶油中脂肪酸及活性成分研究

Research on fatty acids and active components in Camellia oleifera Abel. seed oil of Hunan province

中文关键词:  油茶籽油  脂肪酸  消光系数  多酚  总黄酮

英文关键词:Camellia oleifera Abel. seed oil  fatty acid  extinction coefficient  polyphenols  total flavonoids

中文摘要:

      目的 建立紫外分光光度法快速检测湖南本地油茶(Camellia oleifera Abel.)茶籽油中多酚、总黄酮和消光系数的分析方法。方法 搜集30批次油茶籽油, 建立紫外分光光度法测定油茶籽油的总黄酮值、多酚含量和消光系数, 参照食品安全国家标准, 测定油茶籽油中11种脂肪酸成分。结果 确定紫外分光光度法不同参数指标中茶油待测样品的最大吸收波长, 油茶籽油的总黄酮值含量在0.02~8.32 mg/kg范围之间、多酚含量在7.08~34.63 mg/kg范围之间, 消光系数在0.135~1.445范围之间。11种脂肪酸组成中比例最高的3项依次是油酸、亚油酸和棕榈酸, 不饱和脂肪酸比例超过86%。结论 建立紫外分光光度快速检测法, 有助于对油茶籽油质量分级及定向功能性筛选, 适宜工厂实时监测和化妆品研发数据参考; 对主要脂肪酸进行动脉粥样硬化指数(atherogenic index, AI)和血栓形成指数(thrombosis index, TI)两项营养指数评价, 油茶籽油造成心血管的风险很低, 具有良好的油用价值; 为湖南茶油生产与加工过程中质量安全控制及高附加值产品的研发提供了一定的基础数据。

英文摘要:

      Objective To eatablish a method for the rapid determination of polyphenols, total flavonoids and extinction coefficient in Camellia oleifera Abel. tea seed oil by ultraviolet (UV) spectrophotometry. Methods Thirty batches of Camellia oleifera Abel. seed oil were collected. The total flavonoid value, polyphenol content and extinction coefficient of Camellia oleifera Abel. seed oil were determined by UV spectrophotometry. According to the national food safety standard, 11 fatty acids in Camellia oleifera Abel. seed oil were determined. Results The maximum absorption wavelength of tea oil samples in different parameters of UV spectrophotometry was determined. The content of total flavonoids, polyphenols and extinction coefficient were in the range of 0.02? 8.32 mg/kg, 7.08?34.63 mg/kg and 0.135?1.445, respectively. Among the 11 fatty acids, oleic acid, linoleic acid and palmitic acid had the highest proportion, and unsaturated fatty acid accounted for more than 86%. Conclusion The establishment of a rapid UV spectrophotometry detection method is helpful to the quality classification and directional functional screening of Camellia oleifera Abel. seed oil, which is suitable for real-time monitoring in factories and reference of cosmetics research and development data. The main fatty acids are analyzed by atherosclerosis index (AI) and thrombosis index (TI), the results show that the risk of cardiovascular disease caused by Camellia oleifera Abel. seed oil is very low, and it have good oil value, which provide some basic data for the quality and safety control and the research and development of high value-added products in the production and processing of Camellia oleifera Abel. seed oil in Hunan province.