电喷雾检测器、蒸发光散射检测器与示差折光检测器测定食品中5种糖成分的方法比较

Comparison of determination of 5 carbohydrates in food by charged aerosol detector, evaporative light scattering detector and refractive index detector

中文关键词:  电喷雾检测器  蒸发光散射检测器  示差折光检测器  高效液相色谱法  糖类

英文关键词:charged aerosol detection  evaporative light scattering detection  refractive index detection  high performance liquid chromatography  carbohydrate

中文摘要:

      目的 比较高效液相色谱仪串联电喷雾检测器、蒸发光散射检测器和示差折光检测器测定食品中5种糖分的优缺点。方法 样品依照国家标准进行前处理, 流动相为乙腈-水(75:25, V:V), 经色谱柱Thermo Scientific HYPERSIL APS-2分离, 分别通过3种检测器进行检测, 比较3种检测器检测的标准品线性回归系数、标准曲线最低点信噪比及实际样品的检测结果。结果 电喷雾检测器在标准曲线最低点处信噪比显著优于蒸发光散射检测器和示差折光检测器, 线性回归系数及实际样品检测重现性优于蒸发光散射检测器。结论 电喷雾检测器具备较好的灵敏度、线性及重现性, 具备较好的定量能力, 可作为未来高效液相色谱法检测食品中糖分的备选检测器。

英文摘要:

      Objective To compare the advantages and disadvantages of high performance liquid chromatography with charged aerosol detection, evaporative light scattering detection and refractive index detection for the determination of 5 carbohydrates in food. Methods The samples were prepared by national standard, mobile phase was acetonitrile-water (75:25, V:V), separated by Thermo Scientific HYPERSIL APS-2 column, and detected by charged aerosol detection, evaporative light scattering detection and refractive index detection. The correlation coefficient, S/N ratios and the results of commercial samples were compared. Results The S/N of the charged aerosol detection at the lowest point of the standard curve was significantly better than that of the evaporative light scattering detection and refractive index detection, and the correlation coefficient and results of the commercial samples were significantly better than that of the evaporative light scattering detection. Conclusion Charged aerosol detection has good sensitivity, linearity and reproducibility, and good quantitative ability. It can be used as an alternative detector for the detection of carbohydrates in food in the future.