免疫防御蛋白粉对小鼠免疫功能的影响

Effect of immune defence protein powder on immune function in mice

中文关键词:  免疫防御蛋白粉  免疫调节  小鼠

英文关键词:immune defence protein powder  immunoregulation  mice

中文摘要:

      目的 探究免疫防御蛋白(immune defence proteins, IDP)粉对小鼠免疫功能的影响。方法 将160只无特定病原体(specific pathogen free, SFP)级ICR小鼠随机分为4大组, 每个大组设立0.41、0.83、2.50 g/(kg.BW) 3个剂量组和1个阴性对照组, 实验动物连续经口给予不同剂量受试物30 d后, 分别进行免疫脏器/体重比值测定、细胞免疫功能测定、体液免疫功能测定、单核-巨噬细胞功能测定及NK细胞活性测定。结果 中、高剂量组小鼠足跖肿胀度明显高于对照组, 显示IDP能增强绵羊红细胞(sheep red blood cells, SRBC)诱导的迟发性变态反应; 中、高剂量组小鼠腹腔巨噬细胞的吞噬百分率和吞噬指数均明显高于对照组, 显示IDP可增强巨噬细胞的吞噬能力。结论 IDP具有增强免疫力的作用, 本研究对IDP的进一步开发和应用具有一定的科学价值。

英文摘要:

      Objective To explore the effect of immune defence protein (IDP) powder on the immune function of mice. Methods Total of 160 specific pathogen free (SFP) grade ICR mice were randomly divided into 4 groups, each large group was established with 3 dose groups of 0.41, 0.83, and 2.50 g/(kg.BW) and a negative control group. After the experimental animals were orally administrated with different doses of subjects for consecutive 30 days, their immune organ/body weight ratio, cellular immune function, humoral immune function, monocyte-macrophage function and NK cell activity were measured, respectively. Results The paw edema in the medium and high dose groups was significantly higher than that in the control group, indicating that IDP could enhance the delayed allergic reaction induced by sheep red blood cells (SRBC). The phagocytic percentage and phagocytic index of peritoneal macrophages in the medium and high dose groups were significantly higher than those in the control group, indicating that IDP could enhance the phagocytic ability of macrophages. Conclusion IDP can enhance the immunity, and this study has certain scientific value for the further development and application of IDP.