蛋白质免染系统的应用、常见问题及改进

Application, problems and improvement of protein stain-free system

中文关键词:  免染系统  蛋白质印迹  近红外  3D打印  组织工程

英文关键词:stain-free system  western blotting  near infra-red  3D printing  tissue engineering

中文摘要:

      聚丙烯酰胺凝胶电泳是蛋白质分离的关键方式, 随着蛋白质印迹法的应用, 常被用作生物医学等方面分析的下游工具。近年来, 近红外荧光材料膜的运用以及3D打印等新兴技术逐渐应用于组织工程中, 对蛋白质免染系统提出了更高的定量要求。本文阐述了免染系统的原理, 操作和分析中存在的问题及改进方法, 并对免染技术的发展进行了展望, 以期为实现标准免染技术对蛋白质的准确计量分析提供基础和思路。

英文摘要:

      Polyacrylamide gel electrophoresis is a key method for protein separation. With the application of western blotting, it is often used as a downstream tool for biomedical analysis. In recent years, the application of near-infrared fluorescent material membranes and emerging technologies such as 3D printing have gradually been applied to tissue engineering, putting forward higher quantitative requirements for stain-free systems. This article described the principle of the stain-free system, the problems in its operation and analysis, and its improvement methods. It also looked forward to the development of stain-free technology, hoping to provide the basis and ideas for realizing the accurate measurement and analysis of protein by standard stain-free technology.