抗体芯片技术检测甘薯提取物对结直肠癌荷瘤鼠肝脏免疫细胞因子的影响

Detection of the immune cytokines in the liver of mice bearing the colorectal tumor after sweet potato extract treatment by using the antibody array technology

中文关键词:  甘薯提取物  结直肠癌  免疫  细胞因子  抗体芯片

英文关键词:sweet potato extract  colorectal cancer  immune  cytokine  antibody array

中文摘要:

      目的 研究甘薯提取物(sweet potato extract, SPE)对结直肠癌(colorectal cancer, CRC)荷瘤鼠肝脏中多种免疫细胞因子表达水平的影响。方法 20只雄性Balb/c裸鼠分为2组(对照组、SPE组, 每组10只)。对照组正常饮食、饮水; SPE组经饮水摄入甘薯提取物(平均摄入量2 g/d); 2组均在第5周第1 d腹腔接种人结直肠癌LoVo细胞, 第8周末处死所有动物, 通过抗体芯片技术检测肝组织中多种免疫细胞因子的表达水平。结果 SPE干预使肝组织中c-c基序趋化配体(c-c motif chemokine ligand, CCL)11(P<0.05)、c-x-c基序趋化配体(c-x-c motif chemokine ligand, CXCL)11(P<0.01)、白细胞介素 (interleukin, IL)-10(P<0.05)、IL-12(P<0.05)、IL-22(P<0.01)、IL-23(P<0.05)、基质金属蛋白酶(matrix metalloproteinase, MMP)-2、MMP-3、MMP-9(P<0.01)、双调蛋白和血管生成素样3蛋白表达水平显著降低, 巨噬细胞集落刺激因子(macrophage-colony stimulating factor, M-CSF)(P<0.01)和血管生成素样2蛋白(P<0.05)水平升高, 粒细胞-巨噬细胞集落刺激因子(granulocyte macrophage-colony stimulating factor, GM-CSF)、?干扰素(interferon ? , INF?)、CCL2、CCL3、CCL20、IL-17A、血管生成素样1蛋白无显著改变。结论 经口摄入SPE可降低CRC荷瘤鼠肝组织整体炎症反应, 调节血管生成及组织重塑因子, 有助于恢复肝功能的动态平衡。

英文摘要:

      Objective To research the effects of sweet potato extract (SPE) on the expression of various immune cytokines in the liver of colorectal cancer (CRC) carrying mice. Methods Twenty male Balb/c nude mice were divided into 2 groups (control, SPE, 10 in each group). The control group had normal diet and drinking water; the SPE group received SPE via drinking water (average intake 2 g/d), both groups were intraperitoneally inoculated with human colorectal cancer LoVo cells on the first day of the 5th week, and all animals were sacrificed at the end of the 8th week. The expression of multiple immune cytokines in liver tissues were detected by the antibody array technology. Results SPE significantly reduced the expression of c-c motif chemokine ligand (CCL11) (P<0.05), c-x-c motif chemokine ligand (CXCL11) (P<0.01), interleukin (IL)-10(P<0.05), IL-12(P<0.05), IL-22(P<0.01), IL-23(P<0.05), matrix metalloproteinase(MMP)-2, MMP-3, MMP-9(P<0.01), amphiregulin and angiopoietin like 3, while raised the levels of macrophage-colony stimulating factor (M-CSF) (P<0.01) and angiopoietin-2(P<0.05). The expression of granulocyte macrophage-colony stimulating factor (GM-CSF), interferon ? (INF?), CCL2, CCL3, CCL20, IL-17A, and angiopoietin-1 did not change significantly. Conclusion Oral intake of SPE can reduce the overall inflammatory response level in the liver of CRC tumor-bearing mice,regulate angiogenesis and tissue remodeling factors,which helping restore the homeostasis of liver function.