气相色谱法检测苹果中氟环唑、咪鲜胺及其代谢物的残留

Determination of epoxiconazole, prochloraz and their metabolites residues in apple by gas chromatography

中文关键词:  气相色谱法  咪鲜胺  2,4,6-三氯苯酚  氟环唑  苹果

英文关键词:gas chromatography  prochloraz  2,4,6-trichlorophenol  epoxiconazole  apple

中文摘要:

      目的 建立气相色谱法检测苹果中咪鲜胺及其代谢物2,4,6-三氯苯酚和氟环唑残留的分析方法。方法 匀浆样品加入1%乙酸乙腈振荡, 再加入3 g NaCl、2 g无水Na2SO4高速振荡10 min, 以5000 r/min离心5 min提取, 之后经N-丙基乙二胺(primary secondary amine, PSA)净化, 采用气相色谱法进行检测, 外标法定量。 结果 苹果中3种农药在0.01~2.0 mg/L范围内呈良好线性(r2=0.999)。方法定量限为10~20 μg/kg。平均回收率为74%~108%, 相对标准偏差为2.90%~9.55%。结论 该方法准确、快速、可靠, 适用于苹果中咪鲜胺及其代谢物2,4,6-三氯苯酚和氟环唑残留量的检测。

英文摘要:

      Objective To establish a method for the determination of prochloraz and its metabolites 2,4,6-trichlorophenol and epoxiconazole residues in apples by gas chromatography. Methods The homogenate sample was shaken with 1% acetic acid acetonitrile, and then 3 g NaCl and 2 g anhydrous Na2SO4 were added with 10 min shaking at high speed, centrifuged at 5000 r/min for 5 min, then purified with primary secondary amine (PSA), then detected by gas chromatography, and quantified by external standard. Results The linearities of the three pesticides in apple were good in the concentration range from 0.01?2.0 mg/L(r2=0.999), and the limit of quantification was 10-20 μg/kg. The average recoveries of three pesticides were in the range of 74%-108%, with relative standard deviations 2.90%?9.55%. Conclusion The method has advantages of accurate, rapid and reliable, which can be used to detect the residue of epoxiconazole, prochloraz and 2,4,6-trichlorophenol in apples.