高会兰,潘 舰,韩 叶,朱富强,李艳明.基于保留指数原理结合气相色谱-三重四极杆质谱法快速筛查蔬菜中农药残留的方法研究[J].食品安全质量检测学报,2021,12(2):822-826
基于保留指数原理结合气相色谱-三重四极杆质谱法快速筛查蔬菜中农药残留的方法研究
Rapid screening of pesticide residues in vegetables by gas chromatography tandem mass spectrometry based on retention index
投稿时间:2020-10-12  修订日期:2021-01-06
DOI:
中文关键词:  保留指数  正构烷烃  三重四极杆气相色谱-质谱联用法  农药残留  蔬菜
英文关键词:retention index  n-alkanes  gas chromatography tandem mass spectrometry  pesticide residues  vegetables
基金项目:
作者单位
高会兰 滨州市食品药品检验检测中心 
潘 舰 滨州市食品药品检验检测中心 
韩 叶 滨州市食品药品检验检测中心 
朱富强 滨州市食品药品检验检测中心 
李艳明 滨州市食品药品检验检测中心 
AuthorInstitution
GAO Hui-Lan Binzhou Food and Drug Inspection and Testing Center 
PAN Jian Binzhou Food and Drug Inspection and Testing Center 
HAN Ye Binzhou Food and Drug Inspection and Testing Center 
ZHU Fu-Qiang Binzhou Food and Drug Inspection and Testing Center 
LI Yan-Ming Binzhou Food and Drug Inspection and Testing Center 
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中文摘要:
      目的 建立气相色谱-三重四极杆质谱联用法快速筛查蔬菜中农药残留的方法。方法 利用正构烷烃C9-C33标准溶液, 结合气相色谱-三重四极杆质谱联用仪及其Smart Database Pesticides数据库建立仪器数据采集方法。选用9种农药为目标物, 试样经QuEChERS方法进行萃取净化后, 建立质谱多反应监测(multiple reaction monitoring, MRM)方法, 进行定性、定量分析。结果 采用空白样品添加标准溶液制得质量浓度为0.01、0.05、0.10 mg/kg的样品, 基于保留指数所建立的方法准确定性出待测9种农药, 并测得方法的平均回收率为72.0%~118.8%。结论 该方法简便、快速、成本低, 具有较好的适用性, 对监测农产品中农药的使用种类, 更加全面合理地制定农产品中农残限量范围具有参考价值。
英文摘要:
      Objective To establish a method for rapid screening pesticide residues in vegetables by gas chromatography tandem mass spectrometry. Methods By using C9-C33 n-alkanes standard solution, combined with gas chromatography tandem mass spectrometry and its Smart Database Pesticides to establish the instrument data acquisition method. Nine pesticides were selected as the target, the samples were extracted and purified by QuEChERS method, and multiple reaction monitoring (MRM) method was established for qualitative and quantitative analysis. Results The 0.01, 0.05 and 0.10 mg/kg samples were prepared by adding standard solution to the blank sample. The method based on retention index was used to determine nine pesticides to be tested accurately. The average recovery of the method was 72.0%?118.8%. Conclusion This method is simple, rapid, low cost, and has good applicability. It is of reference value for monitoring the use of pesticides in agricultural products and formulating more comprehensive and reasonable limits of agricultural residues in agricultural products.
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