2019年连云港市农贸市场双壳贝类中诺如病毒污染状况调查

Survey of Norovirus contamination of bivalves mollusks in farmer’s market in Lianyungang city in 2019

中文关键词:  双壳贝类  诺如病毒  基因型

英文关键词:bivalves mollusks  Norovirus  genotype

中文摘要:

      目的 了解连云港市农贸市场双壳贝类中诺如病毒污染状况。方法 于2019年6、8和11月在连云港三县三区各采集10份双壳贝类食物, 共180份, 及时分离消化腺于?75 ℃保存, 采用实时荧光定量PCR检测诺如病毒基因型。结果 5种双壳贝类中诺如病毒基因总检出率为26.67%(48/180), 基因型分型中GI型检出率为5.00%(9/180)、GII型检出率为17.78%(32/180)、GI+GII型检出率为3.89%(7/180); 诺如病毒基因检出率在采样月份、地区分布无差异; 帘蛤科诺如病毒检出率最高。结论 连云港市农贸市场双壳贝类诺如病毒携带形势严峻, 分布特征具有特殊性, 亟需明确本地的诺如病毒传播模式并采取相应防控措施。

英文摘要:

      Objective To investigate the Norovirus contamination in bivalve mollusks in farmers' market in Lianyungang city. Methods A total of 180 samples of bivalve mollusks were collected in various counties and districts of Lianyungang city in June, August and November in 2019, The digestive glands were separated in time and stored at ?75 ℃, and the genotype of Norovirus was detected by real-time fluorescent quantitative PCR. Results The total detection rate of Norovirus from 5 kinds of bivalve mollusks was 26.67% (48/180), the detection rates of type GI, type GII and type GI+GII were 5.00% (9/180), 17.78% (32/180) and 3.89% (7/180). Norovirus gene detection rate had no difference in month and regional distribution. The detection rate of Veneridaeclams Norovirus gene was highest. Conclusion The carrying situation of Norovirus of bivalve mollusks in farmer’s market in Lianyungang is severe, and the distribution characteristics are special. It is urgent to clarify the local Norovirus transmission mode and take corresponding prevention and control measures.