高效液相色谱法测定高温加工食品中丙烯酰胺

Determination of acrylamide in high temperature processed food by high performance liquid chromatography

中文关键词:  高温加工食品  丙烯酰胺  高效液相色谱法

英文关键词:high temperature processed food  acrylamide  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法测定高温加工食品中丙烯酰胺含量的分析方法。方法 样品粉碎后经超纯水提取、正己烷脱脂、C18固相萃取柱萃取、流动相洗脱后得到样品净化液, 经高效液相色谱仪, 以乙腈-水(体积比2:98)作为流动相, 流速为0.8 mL/min, 测定波长205 nm, 柱温20 °C的色谱条件测定, 外标法定量。结果 在该条件下, 丙烯酰胺在0.1~2.0 μg/mL内具有良好的线性关系(r2=0.9987), 检出限14 μg/kg, 定量限40 μg/kg, 加标回收率在91.5%~95.0%之间, 1.0 μg/mL丙烯酰胺标准溶液重复6次进样的相对标准偏差为(relative standard deviation, RSD)为0.16%。结论 此方法可以准确、快速测定高温加工食品中的丙烯酰胺含量。

英文摘要:

      Objective To establish a high performance liquid chromatography method for the determination of acrylamide in high temperature processed food. Methods The sample was extracted by ultra-pure water after crushed, degreased by n-hexane, extracted by C18 solid phase extraction column and eluted by mobile phase. The sample was purified by high performance liquid chromatography with acetonitrile water (2:98, V:V) as mobile phase. The flow rate was 0.8 mL/min, the detection wavelength was 205 nm, and the column temperature was 20 °C. The external standard method was used for quantitative determination. Results Under these conditions, the linear range of acrylamide was 0.1–2.0 μg/mL (r2=0.9987), the limit of detection was 14 μg/kg, the limit of quantitation was 40 μg/kg, the recovery rate was 91.5%–95.0%, and the relative standard deviation (RSD) of 1.0 μg/mL acrylamide standard solution after 6 injections was 0.16% (n=6). Conclusion This method can accurately and quickly determine the content of acrylamide in high temperature processed food.