多重荧光PCR快速检测食源性致病菌的研究进展

Research progress on rapid detection of foodborne pathogens by multiplex fluorescent PCR

中文关键词:  食源性致病菌  多重荧光PCR  快速检测

英文关键词:foodborne pathogens  multiplex fluorescence PCR  rapid detection

中文摘要:

      食源性疾病多由食源性致病菌引发, 威胁个人健康及全球食品安全, 而传统细茵分离、培养与鉴定由于需时较长, 尤其是有的细茵难以培养, 难以适应食源性疾病预防控制的需要, 因此寻找有效、可靠的食源性致病菌高通量检测方法对于确保食品安全具有重要意义。近年来, 食源性致病菌的核酸检测技术具有简便、耗时短、特异性强、灵敏度高等优点, 已被广泛应用于食源性致病菌的检测, 本文在总结食源性致病菌知识的基础上, 着重对多重荧光PCR技术原理及在食源性致病菌检测的应用进展进行综述, 以期为控制由食源性致病菌引发的食品安全隐患提供理论参考。

英文摘要:

      Foodborne diseases are mostly caused by foodborne pathogens, threatening personal health and global food safety. However, due to the long time needed for the separation, culture and identification of traditional bacterial pathogens, some bacterial pathogens are difficult to be cultivated, which is difficult to adapt to the needs of foodborne disease prevention and control. Therefore, it is of great significance to find an effective and reliable high-throughput detection method for foodborne pathogenic bacteria to ensure food safety. In recent years, the nucleic acid detection technology for foodborne pathogenic bacteria has been widely applied in the detection of foodborne pathogenic bacteria due to its advantages of simplicity, short time consuming, strong specificity and high sensitivity. On the basis of summarizing the knowledge of foodborne pathogens, this paper focused on the principle of multiplex fluorescent PCR technology and the application progress in the detection of foodborne pathogens, so as to provide a theoretical reference for the control of food safety hazards caused by foodborne pathogens.