高效液相色谱示差检测法测定饮料中低分子质量抗性麦芽糊精含量

Determination of low molecular weight resistant maltodextrin in beverage by using high-performance liquid chromatography coupled with refractive index detection

中文关键词:  低分子质量抗性麦芽糊精  饮料  高效液相色谱示差检测法  葡萄糖响应因子

英文关键词:low molecular weight resistant maltodextrin  beverage  high performance liquid chromatography with refractive index detection  response factor for dextrose

中文摘要:

      目的 优化标准方法AOAC 2001.03和GB/T 22224-2008《食品中膳食纤维的测定 酶重量法和酶重量法-液相色谱法》的样品前处理过程, 以测定饮料中添加的低分子质量抗性麦芽糊精的含量。方法 样品经一定比例稀释后, 添加丙三醇内标, 经色谱柱分离, 示差折光检测器测定, 内标法定量。结果 方法检出限和定量限分别为0.01 和0.03 g/L, 3个添加水平的平均加标回收率为93.2%~96.9%, 日内相对标准偏差<1.66%, 日间相对标准偏差 <1.81%。同时考察了市场上常见的添加低分子质量抗性麦芽糊精作为膳食纤维的茶饮料, 碳酸饮料和包装饮用水, 测定结果和标签宣称膳食纤维含量基本相符, 且加标回收率为95.9%~102.3%。结论 本方法样品前处理简单、快速, 适用于饮料中添加的低分子质量抗性麦芽糊精含量的测定。

英文摘要:

      Objective To optimize the sample preparation process of standard methods AOAC 2001.03 and GB/T 22224-2008 Determination of dietary fiber in foods-Enzymatic gravimetric method and enzymatic gravimetric method-liquid chromatography, so as to analyze the content of low molecular weight resistant maltodextrin added in beverages. Methods The sample was diluted in a certain proportion and added with glycerol as internal standard, then separated by chromatographic column and analyzed by liquid chromatography coupled with refractive index detection. Results The limits of detection and quantitation were 0.01 and 0.03 g/L, respectively. The average method recoveries were 93.2%?96.9% for the 3 spiking levels, with related standard deviation<1.66% for intra-day and <1.81% for inter-day. Meanwhile, commercial products in different beverage categories (tea, sparkling and package water) were tested, the results were consistent with the labelled dietary fiber value, and the recoveries ranged from 95.9% to 102.3%. Conclusion The method is simple, rapid, accurate and suitable for the determination of fortified low molecular weight resistant maltodextrin in beverages.