食品安全快速检测方法与参比方法一致性的评价方法探讨

Discussion on evaluation method of consistency between the rapid detection methods of food safety and the reference methods

中文关键词:  食品安全快速检测  方法评价  一致性评价  卡方检验  Kappa检验

英文关键词:rapid detection of food safety  method evaluation  evaluation of consistency  chi-square test  Kappa test

中文摘要:

      食品安全快速检测是我国食品安全保障的重要手段, 对食品安全快速检测方法(产品)进行规范化评价, 是保障食品安全快速检测质量的有效手段。目前, 国家相关部门制定的食品安全快速检测方法的评价规范及SN/T 2775-2011《商品化食品检测试剂盒评价方法》对食品安全快速检测方法与参比方法一致性分析都采用了卡方检验。本文对现有一致性分析方法-卡方检验进行了探讨, 指出其局限性(在阳性检测率不变的情况下会随着实验样品数的增加得出相反的结论), 并提出采用Kappa检验替代卡方检验, 并推导简便的计算公式。Kappa检验能够更准确地评价食品安全快速检测方法与参比方法的一致性。

英文摘要:

      Rapid detection of food safety is an important means to ensure food safety in China, standardized evaluation of food safety rapid detection methods or products is an effective means to ensure the quality of food safety rapid detection. At present, chi-square test is used to analyze the consistency between the rapid detection method of food safety and the reference method in evaluation norms formulated by relevant national departments and SN/T 2775-2011 Methods for the evaluation of commercial test kits for food testing purpose. This article discussed the existing consistency analysis method chi-square test, pointed out the limitation of chi-square test (in the case of constant positive detection rate, the opposite conclusion would be drawn with the increase of the number of experimental samples), put forward to use Kappa test instead of chi-square test, and derived the simple calculation formula. The Kappa test can more accurately evaluate the consistency of the food safety rapid detection methods and reference methods.