三通道自动固相萃取-高效液相色谱四极杆串联质谱法检测水中8种痕量微囊藻毒素

Determination of 8 microcystins in the water by tri-channel automatic solid-phase extraction and high performance liquid chromatography- quadrupole tandem mass spectrometry

中文关键词:  三通道自动固相萃取  微囊藻毒素  高效液相色谱-四极杆串联质谱法

英文关键词:three-channel automatic solid phase extraction  microcystins  high performance liquid chromatography-quadrupole tandem mass spectrometry

中文摘要:

      目的 建立三通道自动固相萃取-高效液相色谱-四极杆串联质谱法检测水中痕量的8种微囊藻毒素的分析方法。方法 采用高自动化的前处理设备, 采用C18固相萃取小柱富集浓缩, 应用液相色谱-四极杆串联质谱法定性定量地检测8种微囊藻毒素。结果 该方法检测水中的8种微囊藻毒素检出限为0.01~0.6 μg/L, 在0.25~62.5 μg/L的线性范围中, 线性相关系数大于0.99。结论 该方法自动化程度高、灵敏度高、定性定量准确、测定浓度范围广。

英文摘要:

      Objective To establish a method for determination of 8 microcystins in water by tri-channel automatic solid-phase extraction and high performance liquid chromatography-quadrupole tandem mass spectrometry. Methods The highly automated pre-processing equipment was adopted, the C18 solid phase extraction column was used for enrichment and concentration, and 8 microcystins were quantitatively and qualitatively determined by liquid chromatography-quadrupole tandem mass spectrometry. Results The limits of detection 8 microcystins in water by this method were 0.01?0.6 g/L. The linear correlation coefficient is greater than 0.99 in the linear range of 0.25?62.5 g/L. Conclusion This method has high automatization, high sensitivity, accurate qualitative and quantitative determination and wide concentration determination range.