高效液相色谱法同时测定饮料和果冻中9种食品添加剂

Simultaneous determination of 9 food additives in beverages and jelly by high performance liquid chromatography

中文关键词:  高效液相色谱法  防腐剂  甜味剂  着色剂  饮料  果冻

英文关键词:high performance liquid chromatography  preservatives  sweeteners  colorants  beverages  jelly

中文摘要:

      目的 建立高效液相色谱法同时检测饮料和果冻中9种食品添加剂的分析方法。方法 样品用水提取后, 加入亚铁氰化钾和乙酸锌溶液沉淀蛋白。使用Agilent ZORBAX Eclipse Plus C18色谱柱(250 mm× 4.6 mm, 5 μm), 以甲醇和0.02 mol/L乙酸铵为流动相进行梯度洗脱, 二极管阵列检测器变波长检测, 外标法定量。结果 9种食品添加剂在1.0~100 μg/mL范围内有良好的线性, 相关系数均在0.9995以上, 检出限为0.020~0.431 mg/kg, 定量限为0.068~1.312 mg/kg。在3个水平下加标回收率为80.4%~109.7%, 相对标准偏差低于5%。结论 该方法快速、准确、简便, 适用于饮料及果冻中9种添加剂的检测需求。

英文摘要:

      Objective To establish a method for simultaneous determination of 9 food additives in beverages and jelly by high performance liquid chromatographic. Methods After the sample was extracted with water, ferrous potassium hydride and zinc acetate solution were added to precipitate proteins, and then the solution was gradient eluted by a column of Agilent ZORBAX Eclipse Plus C18 (250 mm×4.6 mm, 5 μm) with the mobile phase of methanol and 0.02 mol/L ammonium acetate solution. Detection was performed using a diode array detector with variable detection wavelengths. The quantification was performed by the external standard method． Results The 9 food additives showed good linearity in the range of 1.0?100 μg/mL, the correlation coefficients were all above 0.9995. The limits of detection were 0.020?0.431 mg/kg, and the limits of quantification were 0.068?1.312 mg/kg. The recoveries of samples at 3 levels were 80.4%?109.7%, and the relative standard deviations were less than 5%. Conclusion This method is simple, rapid and accurate, which is suitable for the detection of 9 additives in beverages or jelly.