高效液相色谱法测定饼干中三氯蔗糖含量

Determination of sucralose content in cookies by high performance liquid chromatography

中文关键词:  饼干  三氯蔗糖  高效液相色谱法

英文关键词:cookies  sucralose  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)检测饼干中三氯蔗糖含量的分析方法。方法 在现行国家标准基础上, 针对饼干样品特点, 优化样品前处理方法, 并进行方法学验证。结果 该方法工作曲线的线性范围为0.02~0.60 mg/mL, 相关系数为0.9999, 回收率为96.78%~100.47%, 峰面积相对标准偏差为0.66% (n=6), 方法检出限为2.0 mg/kg。利用该方法对从市场上随机采购的80份饼干样本进行测定, 标识含有三氯蔗糖的样本均有检出, 未标识含有三氯蔗糖的样本均低于检出限, 按国家标准饼干中最大用量0.25 g/kg的限度要求, 无过量添加。结论 本方法简单、高效、灵敏度和准确度均较高, 适用于检验机构中三氯蔗糖含量的检测。

英文摘要:

      Objective To establish a method for the determination of sucralose in cookies by high performance liquid chromatography (HPLC). Methods According to the characteristics of the cookie samples, the pretreatment method of cookies was optimized based on the current national standard, and the method was experimental validated. Results The linear range were 0.02?0.60 mg/mL, the correlation coefficient was 0.9999, the recoveries of sucralose were 96.78%–100.47%, the relative standard deviation of peak area was 0.66% (n=6), and the limit of detection was 2.0 mg/kg. The method was used to measure 80 biscuit samples randomly purchased from the market. The samples labeled as containing sucralose were all detected, and the samples not labeled as containing sucralose were all below the limit of detection. The maximum dosage of 0.25 g/kg in national standard biscuits was required without excessive addition. Conclusion This method is simple, sensitive and accurate, which is suitable for routine test of sucralose in inspection institutions.