基于适配体检测农药残留的技术进展

Technical progress of pesticide residue detection based on aptamer

中文关键词:  适配体  农药残留  比色法  电化学法

英文关键词:aptamer  pesticide residue  colorimetric method  electrochemical method

中文摘要:

      随着多种农药地广泛使用, 农药残留带来的健康问题越来越受到大众的关注。基于核酸适配体进行农药残留的检测技术在有毒有害物质检测方面发挥着重要作用。相比于抗体, 适配体具有稳定性好、容易合成和修饰、靶标范围广等优势。近些年来, 发表了一些适配体与荧光检测、电化学检测、拉曼光谱等方法进行结合的新型方法。这些方法可以更加准确、灵敏地检测出农药残留量。其中有部分新方法实现了高通量检测。本文总结了近5年使用的核酸适配体进行农药残留检测的方法, 同时比较了前几年的检测方法。

英文摘要:

      With the widespread use of various pesticides, the health problems caused by pesticide residues have attracted more and more public attention. The detection technology of pesticide residues based on nucleic acid aptamers plays an important role in the detection of toxic and hazardous substances. Compared with antibodies, aptamers have the advantages of good stability, easy synthesis and modification, and a wide range of targets. In recent years, some new methods combining aptamer with fluorescence detection, electrochemical detection, Raman spectroscopy and other methods have been published. These methods can detect pesticide residues more accurately and sensitively. Some of the new methods also achieve high-throughput detection. This article summarized the methods of pesticide residue detection using nucleic acid aptamers in the past five years, and compared them with the detection methods of previous years.