高效液相色谱法测定食品中β-阿朴-8’-胡萝卜素醛的含量

Determination of β-apo-8’-carotenal in food by high performance liquid chromatography

中文关键词:  β-阿朴-8’-胡萝卜素醛  高效液相色谱法  食品添加剂  着色剂

英文关键词:β-apo-8’-carotenal  high performance liquid chromatography  food additives  colorants

中文摘要:

      目的 建立高效液相色谱法测定多种食品中β-阿朴-8’-胡萝卜素醛的含量的方法。方法 样品经20%氢氧化钾40 ℃皂化30 min后, 用正己烷提取, 浓缩蒸干后经Phenomenonex Luna C18色谱柱分离, 以乙腈-水(95:5, V:V)为流动相等度洗脱, 在二极管阵列检测器460 nm条件下检测, 外标法定量。结果 β-阿朴-8’-胡萝卜素醛在0.05~1 μg/mL质量浓度范围内线性关系良好, 相关系数r2＞0.999, 检出限为0.0002 g/kg, 回收率为92.3%~97.0%, 相对标准偏差为2.0%~4.7%(n=6)。结论 本方法检出限与定量限均可以满足国家标准中的限量要求, 适用于多种食品中β-阿朴-8’-胡萝卜素醛的含量的检测。

英文摘要:

      Objective To establish a method for the determination of β-apo-8’-carotenal in food by high performance liquid chromatography. Methods The samples were saponified with 20% potassium hydroxide at 40 ℃ for 30 min, then extracted with n-hexane, concentrated and evaporated to dryness, and then separated with Phenomenonex Luna C18 column using acetonitrile-water (95:5, V:V) as mobile phase for isocratic elution, detected by a diode array detector at 460 nm, and quantified by external standard method. Results β-apo-8’-carotenal had a good linear relationship within the mass concentration range of 0.05?1 μg/mL, and the correlation coefficient r2 was more than 0.999. The limit of detection was 0.0002 g/kg, the recoveries were 92.3%?97.0%, and the relative standard deviations were 2.0%?4.7% (n=6). Conclusion The limit of detection and limit of quantification of this method can meet the limit requirements in the national standard, and it is suitable for the detection of β-apo-8’-carotenal content in various foods.