顶空/气相-质谱法测定蔬菜中顺(反)-1,3-二氯丙烯含量

Determination of cis(trans)-1,3-dichloropropene in vegetable by headspace gas chromatography-mass spectrometry

中文关键词:  1,3-二氯丙烯  蔬菜  气相色谱-质谱法  顶空

英文关键词:1,3-dichloropropene  vegetable  gas chromatography-mass spectrometry  headspace

中文摘要:

      目的 建立顶空/气相色谱质谱法测定蔬菜中顺(反)-1,3-二氯丙烯含量。方法 样品中加入饱和NaCl溶液(磷酸调pH小于2), 利用顶空/气相色谱质谱法检测顺(反)-1,3-二氯丙烯含量。结果 在质量浓度为0.1～10 μg/L范围内, 顺(反)-1,3-二氯丙烯线性相关系数r2分别为0.9999、0.9997, 检出限为2 μg/kg。顺(反)-1,3-二氯丙烯添加水平在0.4~40 μg/kg范围内, 6次重复的平均添加回收率分别为93.98%~117.68%和93.92%~98.23%, 相对标准偏差分别为3.49%~8.14%和1.15%~9.72%。结论 该方法准确度高、精密度良好, 符合分析测试质控要求。

英文摘要:

      Objective To establish a method for the determination of cis (trans)-1,3- dichloropropene in vegetables by headspace gas chromatography-mass spectrometry. Methods Saturated NaCl solution (pH adjusted by phosphoric acid was less than 2) was added into the sample, and the content of cis(trans)-1, 3-dichloropropene was detected by headspace gas chromatography-mass spectrometry. Results When the concentration range was 0.1? 10 μg/L, the correlation coefficient of cis (trans)-1,3- dichloropropene were 0.9999 and 0.9997 respectively, and the limit of detection was 2 μg/kg. The average recoveries were 93.98%?117.68% and 93.92%-98.23%, and the relative standard deviations were 3.49%?8.14% and 1.15%?9.72% respectively, at fortified concentration range of 0.4? 40 μg/kg. Conclusion This method has high accuracy and good precision, which meets the requirements of quality control of analysis and test.