武汉地区食源性沙门氏菌耐药特征分析

Analysis of drug resistance characteristics of food-borne Salmonella in Wuhan area

中文关键词:  沙门氏菌  耐药性  耐药基因

英文关键词:Salmonella  drug resistance  drug resistance gene

中文摘要:

      目的 分析武汉市售肉类中分离的82株沙门氏菌的药物敏感性及耐药基因。方法 根据美国临床和实验室标准协会(Clinical & Laboratory Standards Institute, CLSI)相应标准获得沙门氏菌耐药实验种类和浓度, 定制药敏板, 采用最低抑菌浓度法(minimum inhibitory concentration, MIC)进行药敏实验, 并通过PCR方法检测耐药基因的携带情况。结果 药物敏感性试验结果显示, 82株沙门氏菌对四环素耐药率最高, 有66株菌株对四环素耐药, 耐药率高达79.52%, 对复方磺胺和氨苄西林的耐药率较高, 耐药率分别为49.40%和48.19%; 对头孢类药物的耐药率较低, 对亚胺培南未表现出耐药性。耐药基因检测结果显示, 82株沙门氏菌中四环素耐药基因tetA的检出率最高, 且有54株沙门氏菌携带多重耐药基因, 头孢类药物耐药基因blaCTX检出率较低, 仅为3.66%。结论 武汉市食源性沙门氏菌耐药情况较为严重, 部分常见抗生素耐药性较高, 对临床用药治疗和食源性病菌预防造成巨大的影响。

英文摘要:

      Objective To analyze the drug susceptibility and drug resistance genes of 82 strains of Salmonella isolated from meat sold in Wuhan. Methods According to the corresponding standards of the Clinical & Laboratory Standards Institute (CLSI), the type and concentration of Salmonella resistance test were obtained, the drug susceptibility plate was customized, the drug susceptibility experiments was performed by minimum inhibitory concentration (MIC) method, and the carrying status of drug-resistant genes was detected by PCR. Results The results of the drug susceptibility test showed that 82 strains of Salmonella had the highest resistance rate to tetracycline, 66 strains were resistant to tetracycline, the resistance rate was as high as 79.52%, and the resistance rate to compound sulfa and ampicillin was higher, and the resistance rates were 49.40% and 48.19%, respectively. The resistance rate to cephalosporins was low, and none strain of Salmonella was no resistance to imipenem. The results of drug resistance gene detection showed that the tetracycline resistance gene tetA had the highest detection rate among 82 Salmonella strains, and 54 Salmonella strains carried multiple drug resistance genes. The detection rate of the cephalosporin drug resistance gene blaCTX was low, only 3.66%. Conclusion The drug resistance of food-borne Salmonella in Wuhan is relatively serious, and some common antibiotics are highly resistant, which has a huge impact on clinical medication and prevention of food-borne bacteria.