金黄色葡萄球菌定性定量检测中显色培养基的效果评价

Evaluation of chromogenic medium in qualitative and quantitative detection of Staphylococcus aureus

中文关键词:  金黄色葡萄球菌  显色培养基  Baird-Parker培养基  定性定量法  全自动微生物鉴定药敏分析系统

英文关键词:Staphylococcus aureus  chromogenic medium  Baird-Parker medium  qualitative and quantitative method  automatic microbial identification and drug sensitivity analysis system

中文摘要:

      目的 评估显色培养基对金黄色葡萄球菌定性定量的检测效果。方法 金黄色葡萄球菌标准菌株、食品样本分离菌株(30株金黄色葡萄球菌、30株其他葡萄球菌, 5株常见食源性致病菌)分别用金黄色葡萄球菌显色培养基和Baird-Parker培养基进行培养实验, 并用国标法和全自动微生物鉴定药敏分析系统对培养结果进行确证鉴定。结果 实验定性结果显示金黄色葡萄球菌在显色培养基上呈粉紫色, 30株其他葡萄球菌呈蓝色或乳白色, 5株常见食源性致病菌其中单核细胞增生李斯特氏菌呈蓝绿色、表皮葡萄球菌呈乳白色、其余3株不生长, 可在直观上对几种食品中常见葡萄球菌进行有效区别, 而且目标菌在显色培养基和Baird-Parker培养基上计数值无显著差异(P＞0.05), 确证结果显示全自动微生物鉴定药敏分析系统结果与GB 4789.10-2016方法结果一致。结论 显色培养基能快速锁定疑似目标菌落, 为溯源调查指明方向, 定量计数直观有效, 配合全自动微生物鉴定药敏分析系统更提高了对目标菌的鉴定效率, 更适用于金黄色葡萄球菌定性定量检测。

英文摘要:

      Objective To evaluate the qualitative and quantitative detection of Staphylococcus aureus by chromogenic medium. Methods The standard strains of Staphylococcus aureus and the isolated strains of food samples (30 strains of Staphylococcus aureus, 30 other strains of Staphylococcus aureus, and 5 common foodborne pathogens) were cultured on Staphylococcus aureus chromogenic medium and Baird-Parker medium, respectively. The culture results were confirmed and identified by national standard method and automatic microbial identification drug sensitivity analysis system. Results The qualitative results showed that Staphylococcus aureus was powdery purple on the chromogenic medium, 30 strains of other Staphylococcus were blue or opalescent, 5 strains of common foodborne pathogens were Listeria monocytogenes were blue-green, Staphylococcus epidermidis were opalescent, and the remaining 3 strains did not grow, which could visually distinguish the common Staphylococcus in several foods effectively, and there was no significant difference in the counting between the target bacteria on the chromogenic medium and the Baird-Parker medium (P>0.05). The confirmative results showed that the results of the automatic microbial identification and drug sensitivity analysis system were consistent with the results of the GB 4789.10-2016 method. Conclusion The chromogenic medium can quickly locate the suspected bacterial colonies, be easy to traceability investigate, and effectively and quantitatively count the colonies. With the automatic microbial identification and drug sensitivity analysis system, the identification efficiency of the target bacteria is improved, and it is more suitable for the qualitative and quantitative detection of Staphylococcus aureus.