超高效液相色谱-串联质谱法检测预包装畜禽肉制品中多种兽药残留

Determination of veterinary drug residues in prepackaged animal and poultry meat products by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  预包装畜禽肉制品  兽药残留  超高效液相色谱-串联质谱法

英文关键词:prepackaged meat products  veterinary drug residues  ultra performance liquid chromate graphy-tandem mass spectrometry

中文摘要:

      目的 建立超高效液相色谱-串联质谱法检测预包装畜禽肉制品中54种兽药残留的方法。方法 样品经甲酸乙腈提取, Oasis PRiME HLB固相萃取柱净化, 经BEH C18色谱柱分离, 以甲醇和0.1％甲酸水溶液为流动相进行梯度洗脱, 电喷雾正离子模式电离, 外标法定量。结果 54种化合物在0.5～50 ng/mL范围内均呈现良好的线性关系, 相关系数均大于0.990, 检出限0.03～3.51 μg/kg, 回收率为61.4%～97.4%, 相对标准偏差为4.5%～13.3%。结论 本方法操作简便, 稳定性好, 适用于预包装畜禽肉制品中54种兽药残留的同时定性、定量分析。

英文摘要:

      Objective To establish a method for the determination of 54 kinds of veterinary drug residues in prepackaged animal and poultry meat products by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The samples were extracted by formic acid and acetonitrile, purified by Oasis PRiME HLB solid phase extraction column, separated with BEH C18 chromatographic column for gradient elution using methanol and 0.1% formic acid aqueous solution as mobile phase, ionized by electrospray positive ion mode, and quantified by external standard method. Results The 54 compounds showed good linear relationships in the range of 0.5?50 ng/mL, the correlation coefficients were all greater than 0.990, the limits of detection were 0.03?3.51 μg/kg, the recovery rates were 61.4%?97.4%, and the relative standard deviations were 4.5%?13.3%. Conclusion The method is simple and stable, and suitable for the simultaneous qualitative and quantitative analysis of 54 kinds of veterinary drug residues in prepackaged animal and poultry meat products.