杏鲍菇多肽的制备及体外活性研究

Study on the preparation and in vitro activity of polypeptides from Pleurotus eryngii

中文关键词:  杏鲍菇  多肽制备  体外活性

英文关键词:Pleurotus eryngii  preparation of polypeptides  in vitro activity

中文摘要:

      目的 制备杏鲍菇多肽, 并研究其体外活性。方法 以杏鲍菇菇脚为原料, 采用超声辅助的碱提酸沉法提取出杏鲍菇蛋白, 比较3种蛋白酶酶解后的多肽得率, 选定水解酶。基于单因素实验、正交实验确定多肽最优酶解工艺, 并依托体外抗氧化活性实验实现对多肽体外活性的测定。结果 杏鲍菇多肽液在分子量为5~10 kD、1~5 kD及低于1 kD的3种情况下均可成功还原铁离子, 具备抗氧化能力, 同时可清除?OH与DPPH?自由基, 多肽溶液浓度越高、清除效果越好。结论 本研究可充分利用杏鲍菇菇脚, 形成高效的杏鲍菇蛋白提取、酶解以及多肽制备工艺, 有助于进一步提升杏鲍菇的利用价值与经济效益, 并且能够为多肽保健品的制备工艺研究提供重要借鉴意义。

英文摘要:

      Objective To prepare polypeptides of Pleurotus eryngii and study their activity in vitro. Methods The protein was extracted from the foot of Pleurotus eryngii by ultrasonic assisted alkali extraction and acid precipitation. The polypeptide yield of 3 proteases was compared and the hydrolase was selected. Based on single factor experiment and orthogonal experiment, the optimal enzymatic hydrolysis process of polypeptides was obtained, and the in vitro activity of polypeptides was determined by antioxidant activity experiment. Results Under the molecular weight of 5?10 kD, 1?5 kD and less than 1 kD, the polypeptide solution of Pleurotus eryngii was able to reduce the iron ion and had the antioxidant ability. At the same time, the scavenging effect of ?OH and DPPH? free radicals was better with higher concentration of polypeptide solution. Conclusion This study can make full use of the foot of Pleurotus eryngii to form efficient protein extraction, enzymolysis and polypeptide preparation technology, which is helpful to further enhance the utilization value and economic benefits of Pleurotus eryngii, and can provide important reference for the research on the preparation technology of polypeptide health products